One-Step Genetic Modification by Embryonic Doral Aorta Injection of Adenoviral CRISPR/Cas9 Vector in Chicken
文献类型： 外文期刊
作者： Qin, Chao 1 ; Jiang, Shengyao 1 ; Xu, Ke 1 ; Zhu, Jianshen 1 ; Wang, Liyuan 1 ; Yang, Wenhao 1 ; Xiao, Fuquan 1 ; Yang, Kaixuan 2 ; Huang, Qizhong 2 ; Meng, He 1 ;
作者机构： 1.Shanghai Jiao Tong Univ, Sch Agr & Biol, Dept Anim Sci, Shanghai Key Lab Vet Biotechnol, Shanghai 200240, Peoples R China
2.Shanghai Acad Agr Sci, Anim Husb & Vet Res Inst, Shanghai 200030, Peoples R China
关键词： chicken; CRISPR/Cas9; dorsal aorta; adenovirus
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）
ISSN： 1661-6596
年卷期： 2024 年 25 卷 16 期
页码：
收录情况： SCI
摘要： In the avian species, genetic modification by cell nuclear transfer is infeasible due to its unique reproductive system. The in vitro primordial germ cell modification approach is difficult and cumbersome, although it is the main method of genetic modification in chickens. In the present study, the adenoviral CRISPR/Cas9 vector was directly microinjected into the dorsal aorta of chicken embryos to achieve in vivo genetic modification. The results demonstrated that keratin 75-like 4 (KRT75L4), a candidate gene crucial for feather development, was widely knocked out, and an 8bp deletion was the predominant mutation that occurred in multiple tissues in chimeras, particularly in the gonad (2.63-11.57%). As we expected, significant modification was detected in the sperm of G0 (0.16-4.85%), confirming the potential to generate homozygous chickens and establishing this vector as a simple and effective method for genetic modification in avian species.
- 相关文献
作者其他论文 更多>>
-
Intergenerational Transmission of Gut Microbiome from Infected and Non-Infected Salmonella pullorum Hens
作者：Niu, Qing;Yang, Kaixuan;Zhou, Zhenxiang;Huang, Qizhong;Niu, Qing;Yang, Kaixuan;Zhou, Zhenxiang;Huang, Qizhong;Wang, Junliang
关键词：poultry; Salmonella pullorum; fecal microbiota transplantation; microbial structure; prediction functions
-
The host susceptibility/resistance-related genes and gut microbial characteristics in Salmonella pullorum-infected chickens
作者：Ding, Jinmei;Zhu, Jianshen;Zhou, Hao;Qin, Chao;Han, Chengxiao;Yang, Lingyu;He, Chuan;Xu, Ke;Zheng, Yuming;Luo, Huaixi;Chen, Kangchun;Zhou, Wenchuan;Jiang, Shengyao;Liu, Jiajia;Zhu, Wenqi;Meng, He;Yang, Kaixuan;Niu, Qing;Zhou, Zhenxiang;Huang, Qizhong;Zhang, Yaodong;Wang, Shaohui;Yu, Shengqing
关键词：pullorum disease; Salmonella pullorum; gut microbiota; susceptibility/resistance; genome-wide association analysis
-
Near telomere-to-telomere assembly of the Tarim pigeon (Columba livia) genome
作者：Yang, Wenhao;Zhou, Hao;Huang, Jinlong;Zhu, Wenqi;Liu, Jiajia;Qin, Chao;Wang, Liyuan;Luo, Huaixi;Zhu, Jianshen;Xiao, Fuquan;Meng, He;Huang, Jinlong;Hou, Haobin;Yao, Junfeng;Yang, Changsuo;Li, Hejun;Zhao, Lele;Zhang, Jiang
关键词：
-
Microbiome and Microbial Pure Culture Study Reveal Commensal Microorganisms Alleviate Salmonella enterica Serovar Pullorum Infection in Chickens
作者：Zhu, Jianshen;Ding, Jinmei;Zhou, Hao;Yang, Wenhao;Qin, Chao;Wang, Liyuan;Xiao, Fuquan;Meng, He;Yang, Kaixuan;Niu, Qing;Zhou, Zhenxiang;Huang, Qizhong;Zhang, Beibei;Yu, Shengqing;Wang, Shaohui
关键词：pullorum disease; Salmonella pullorum; chicken gut microbiota; Lacticaseibacillus
-
Identification of the gut microbiota affecting Salmonella pullorum and their relationship with reproductive performance in hens
作者：Niu, Qing;Yang, Kaixuan;Gu, Caiju;Zhou, Zhenxiang;Huang, Qizhong;Wang, Xiaoxu;Qi, Xinyong;Cao, Changjian
关键词：gut microbiota; poultry; pullorum; microbial structure; prediction functions
-
How Has the COVID-19 Pandemic Changed Urban Consumers' Ways of Buying Agricultural Products? Evidence from Shanghai, China
作者：Liu, Zengjin;Zhou, Zhou;Wang, Liyuan;Zhao, Jing;Yu, Zhuo;Chen, Yusheng
关键词：COVID-19 pandemic; consumer behavior; consumption channel of agricultural products; influence factors
-
Food Security and Climate Stabilization: Can Cereal Production Systems Address Both?
作者：Liang, Long;Ridoutt, Bradley G.;Ridoutt, Bradley G.;Wang, Liyuan
关键词：agricultural soils; GHG emission; life cycle assessment; product carbon footprint; carbon efficiency; agricultural intensification; fertilizer management
