您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Long-Residence Pneumonia Vaccine Developed Using PEG-Grafted Hybrid Nanovesicles from Cell Membrane Fusion of Mycoplasma and IFN-gamma-Primed Macrophages

文献类型： 外文期刊

作者： Zhang, Zhenzhen 1 ; Wang, Haiyan 1 ; Xie, Xing 1 ; Chen, Rong 1 ; Li, Jun 1 ; Ni, Bo 1 ; Yu, Pei 2 ; Liu, Zunfeng 3 ; Shao, 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing 210014, Peoples R China

2.China Pharmaceut Univ, Sch Tradit Chinese Pharm, Nanjing 210009, Peoples R China

3.Nankai Univ, State Key Lab Med Chem Biol, Key Lab Funct Polymer Mat, Coll Pharm, Tianjin 300071, Peoples R China

4.China Pharmaceut Univ, Dept Sci, Nanjing 211198, Peoples R China

关键词： CD8; (+) T cell responses; cell membrane fusion; hybrid nanovesicles; macrophages; pneumonia vaccine

期刊名称：SMALL （ 影响因子：13.281； 五年影响因子：12.463 ）

ISSN： 1613-6810

年卷期： 2021 年 17 卷 34 期

页码：

收录情况： SCI

摘要： CD8(+) T cell responses play a critical regulatory role in protection against mycoplasma infection-related respiratory diseases. Nanovesicles derived from cell membranes have been shown to induce CD8(+) T cell responses. Moreover, the short residence time of mycoplasma membrane-related vaccines in local lymph nodes limits the efficacy of current mycoplasma vaccines. Here, a long-residence pneumonia vaccine is developed using nanovesicles prepared by cell membrane fusion of Mycoplasma hyopneumoniae and interferon-gamma (IFN-gamma -)-primed macrophages, which are grafted with polyethylene glycol to increase residence time in the lymph nodes. Upregulation of intercellular adhesion molecule-1 (ICAM-1) on the membrane of IFN-gamma-primed macrophages increases the targeting of the hybrid nanovesicle vaccine to the local lymph nodes, with increased CD8(+) T cell activation. A mechanistic study reveals that CD8(+) T cell activation is achieved via a pathway involving upregulation of C-C motif chemokine ligand 2/3 expression by E26 transformation-specific sequences, followed by increased immune-stimulatory activity of dendritic cells. In vivo, prophylactic testing reveals that the hybrid nanovesicle vaccine triggers a long-term immune response, as evidenced by a memory CD8(+) T cell response against mycoplasma infection. The current study provides a new design strategy for mycoplasma vaccines that involves a hybrid method using biological sources and artificial modification.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台