Identification and characterization of circRNAs in peri-implantation endometrium between Yorkshire and Erhualian pigs
文献类型： 外文期刊
作者： Zhou, Chen 1 ; Cheng, Xinyan 1 ; Meng, Fanming 3 ; Wang, Yongzhong 1 ; Luo, Wanyun 1 ; Zheng, Enqin 1 ; Cai, Gengyuan 1 ; Wu, Zhenfang 1 ; Li, Zicong 1 ; Hong, Linjun 1 ;
作者机构： 1.South China Agr Univ, Coll Anim Sci, Natl Engn Res Ctr Breeding Swine Ind, Guangzhou 510642, Peoples R China
2.South China Agr Univ, Guangdong Prov Key Lab Agroanim Genom & Mol Breedi, Guangzhou 510642, Peoples R China
3.Guangdong Acad Agr Sci, Inst Anim Sci, Guangdong Key Lab Anim Breeding & Nutr, Guangzhou 510640, Peoples R China
4.State Key Lab Swine & Poultry Breeding Ind, Guangzhou 510640, Peoples R China
5.Subctr Guangdong Lab Lingnan Modern Agr, Yunfu 527300, Peoples R China
关键词： CircRNA; Erhualian; Yorkshire; Peri-Implantation; Endometrium; Embryo implantation
期刊名称：BMC GENOMICS （ 影响因子：4.4； 五年影响因子：4.7 ）
ISSN： 1471-2164
年卷期： 2023 年 24 卷 1 期
页码：
收录情况： SCI
摘要： BackgroundOne of the most critical periods for the loss of pig embryos is the 12th day of gestation when implantation begins. Recent studies have shown that non-coding RNAs (ncRNAs) play important regulatory roles during pregnancy. Circular RNAs (circRNAs) are a kind of ubiquitously expressed ncRNAs that can directly regulate the binding proteins or regulate the expression of target genes by adsorbing micro RNAs (miRNA).ResultsWe used the Illumina Novaseq6,000 technology to analyze the circRNA expression profile in the endometrium of three Erhualian (EH12) and three Yorkshire (YK12) pigs on day 12 of gestation. Overall, a total of 22,108 circRNAs were identified. Of these, 4051 circRNAs were specific to EH12 and 5889 circRNAs were specific to YK12, indicating a high level of breed specificity. Further analysis showed that there were 641 significant differentially expressed circRNAs (SDEcircRNAs) in EH12 compared with YK12 (FDR < 0.05). Functional enrichment of differential circRNA host genes revealed many pathways and genes associated with reproduction and regulation of embryo development. Network analysis of circRNA-miRNA interactions further supported the idea that circRNAs act as sponges for miRNAs to regulate gene expression. The prediction of differential circRNA binding proteins further explored the potential regulatory pathways of circRNAs. Analysis of SDEcircRNAs suggested a possible reason for the difference in embryo survival between the two breeds at the peri-implantation stage.ConclusionsTogether, these data suggest that circRNAs are abundantly expressed in the endometrium during the peri-implantation period in pigs and are important regulators of related genes. The results of this study will help to further understand the differences in molecular pathways between the two breeds during the critical implantation period of pregnancy, and will help to provide insight into the molecular mechanisms that contribute to the establishment of pregnancy and embryo loss in pigs.
- 相关文献
作者其他论文 更多>>
-
Analysis of differential transcriptome expression reveals that ISG15 provides support for embryo development by promoting angiogenesis in porcine mesometrium
作者：Gan, Jianyu;Wang, Yongzhong;Zhang, Fan;Zang, Xupeng;Gu, Ting;Xu, Zheng;Li, Zicong;Cai, Gengyuan;Wu, Zhenfang;Hong, Linjun;Gan, Jianyu;Wang, Yongzhong;Zhang, Fan;Zang, Xupeng;Gu, Ting;Xu, Zheng;Li, Zicong;Cai, Gengyuan;Wu, Zhenfang;Hong, Linjun;Meng, Fanming;Wu, Zhenfang;Hong, Linjun
关键词：Pig; Mesometrium; Transcriptome; Interferon response; ISG15
-
Characterization of lipids in Guangdong small-ear spotted pig and their crossbreeds during cold storage revealed by LC-MS-based method
作者：Huang, Wenyi;Li, Linfeng;Wang, Pan;Liu, Huilin;Liu, Midi;Meng, Fanming;Cheng, Leiyan;Hu, Bin;Zeng, Jianhua;Yang, Linfang
关键词：Pork; Cold storage; Lipidomics; Differential lipids; Meat quality
-
Analysis of Transcriptome Differences Between Subcutaneous and Intramuscular Adipose Tissue of Tibetan Pigs
作者：Li, Xinming;Huang, Qiuyan;Meng, Fanming;Hong, Chun;Li, Baohong;Yang, Yecheng;Qu, Zixiao;Wu, Junda;Li, Fei;Xin, Haiyun;Hu, Bin;Wu, Jie;Du, Zongliang;Wang, Sutian;Huang, Qiuyan;Hu, Chuanhuo;Zhu, Xiangxing;Tang, Dongsheng
关键词：Tibetan pig; fat deposition; subcutaneous fat; intramuscular fat; RNA-seq
-
GPCPD1 is regulated by METTL3/IGF2BP3 dependent m6A modification to affect pig muscle fiber type conversion
作者：Yang, Yuming;Xiao, Liyao;Huang, Huijun;Su, Jiawei;Hong, Linjun;Cai, Gengyuan;Li, Zicong;Gu, Ting;Yang, Yuming;Xiao, Liyao;Huang, Huijun;Su, Jiawei;Hong, Linjun;Cai, Gengyuan;Li, Zicong;Gu, Ting;Tan, Baohua;Xu, Weihua;Zhao, Yaolu;Dai, Lihe
关键词：GPCPD1; Pig; m 6 A; Myogenic differentiation; Muscle fiber type conversion
-
Unveiling the Regulatory Mechanism of Tibetan Pigs Adipogenesis Mediated by WNT16: From Differential Phenotypes to the Application of Multi-Omics Approaches
作者：Huang, Qiuyan;Meng, Fanming;Hong, Chun;Li, Xinming;Li, Baohong;Wu, Jie;Xin, Haiyun;Wang, Sutian;Zhang, Kunli;Lin, Sen;Hu, Chuanhuo;Zhu, Xiangxing;Tang, Dongsheng;Pei, Yangli
关键词：pigs; IMF; transcriptome; metabolome; fat deposition
-
Genome-Wide Association Study Reveals Genetic Mechanisms Underlying Intersex and Aproctia in Large White Pigs
作者：Li, Yajun;Shi, Jiaxin;Yang, Yingshan;Ruan, Donglin;Wu, Jie;Lin, Danyang;Liao, Zihao;Hong, Xinrun;Zhou, Fuchen;Liu, Langqing;Yang, Jie;Zheng, Enqin;Wu, Zhenfang;Cai, Gengyuan;Zhang, Zebin;Li, Yajun;Shi, Jiaxin;Yang, Yingshan;Ruan, Donglin;Wu, Jie;Lin, Danyang;Liao, Zihao;Hong, Xinrun;Zhou, Fuchen;Liu, Langqing;Yang, Jie;Zheng, Enqin;Wu, Zhenfang;Cai, Gengyuan;Zhang, Zebin;Wu, Jie;Yang, Ming;Wu, Zhenfang;Cai, Gengyuan;Wu, Zhenfang;Cai, Gengyuan
关键词：intersex; aproctia; chip data; imputed data; mixed linear models; genome-wide association studies
-
TLR4 signalling in ischemia/reperfusion injury: a promising target for linking inflammation, oxidative stress and programmed cell death to improve organ transplantation outcomes
作者：Wang, Sutian;Huang, Qiuyan;Meng, Fanming;Zhang, Kunli;Deng, Shoulong;Deng, Shoulong
关键词：toll-like receptor 4; ischemia/reperfusion injury; organ transplantation; inflammation; oxidative stress; programmed cell death
