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Molecular cloning of the HGD gene and association of SNPs with meat quality traits in Chinese red cattle

文献类型： 外文期刊

作者： Zhou, Guoli 4 ; Dudgeon, Crissy 3 ; Li, Mei 2 ; Cao, Yang 1 ; Zhang, Lichun 1 ; Jin, Haiguo 1 ;

作者机构： 1.Jilin Acad Agr Sci, Branch Anim Husb, Gongzhuling 136100, Peoples R China

2.Univ Pittsburgh, Dept Pathol, Pittsburgh, PA 15213 USA

3.Univ Pittsburgh, Dept Pharmacol & Chem Biol, Pittsburgh, PA 15213 USA

4.Liaocheng Univ, Coll Life Sci, Liaocheng 252059, Peoples R China

5.NE Agr Univ, Coll Anim Sci & Techol, Harbin 150030, Peoples R China

关键词： HGD gene;Single nucleotide polymorphisms;Meat quality traits;Cattle;Association analysis

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

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收录情况： SCI

摘要： Homogentisate 1, 2 dioxygenase (HGD) is one of six enzymes required for the catabolism of the aromatic amino acids phenylalanine and tyrosine. Here we present the nucleotide sequence of transcripts of the bovine HGD gene. The full length cDNA of bovine HGD has been identified, encoding a deduced protein of 445 amino acids (Accession No. FJ515744). The bovine HGD gene comprises 14 exons and 13 introns. This is the first published cDNA bovine sequences that share high sequence similarity with other species. Semi-quantitative RT-PCR analysis demonstrated that the bovine HGD transcript was mainly expressed in liver and kidney tissues. Nine single nucleotide polymorphisms (SNPs) were identified, five in the coding region and four intronic. Four of the SNPs change an amino acid in the HGD protein sequence. Genotype and allelic frequencies were determined in Chinese red cattle breeds. Ten haplotypes were determined based upon the genotype of 9 SNPs. Moreover, for the first time an association was reported between HGD gene polymorphism and meat quality traits in Chinese red cattle (n = 224). Marker-trait association analyses showed that the HGD/PvuII genotypes showed a significant effect on meat cooking rate, muscle fiber diameter, and shear force (P < 0.05). The HGD DraIII genotypes showed a significant effect on muscle fiber diameter, shear force, and drip loss (P < 0.05). The HGD/AluI genotypes showed a significant effect on meat cooking rate, shear force, and drip loss (P < 0.05). The HGD/DraI genotypes showed a significant effect on meat cooking rate and shear force (P < 0.05). The HGD/EcoRV genotypes showed a significant effect on meat cooking rate, muscle fiber diameter, and shear force (P < 0.05). In all loci, no statistically significant differences were observed for pHu (P > 0.05). This is the first incidence were polymorphisms of a bovine HGD gene have demonstrated a correlation with meat traits in Chinese red cattle.

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