文献类型： 外文期刊
作者： Li, Bo 1 ; Zheng, Shucheng 5 ; Wang, Yingying 1 ; Wang, Qing 1 ; Li, Yingying 1 ; Yin, Jiyuan 1 ; Ren, Yan 1 ; Shi, Cunbin 1 ; Zhao, Zhiying 4 ; Jiang, Zhiyong 7 ; Bergmann, Sven M. 6 ; Zeng, Weiwei 3 ;
作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Fishery Drug Dev, Key Lab Aquat Anim Immune Technol Guangdong Prov,M, Guangzhou, Guangdong, Peoples R China
2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
3.Foshan Univ, Sch Life Sci & Engn, Guangdong Prov Key Lab Anim Mol Design & Precise B, Foshan, Peoples R China
4.Hainan Acad Ocean & Fisheries Sci, Haikou 571126, Peoples R China
5.Xiamen Univ, Coll Ocean & Earth Sci, Xiamen, Fujian, Peoples R China
6.Friedrich Loeffler Inst FLI, Fed Res Inst Anim Hlth, Inst Infectol, Greifswald Insel Riems, Germany
7.Guangdong Prov Agr Technol Extens Ctr, Guangzhou 510030, Guangdong, Peoples R China
8.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou, Peoples R China
关键词： Tilapia lake virus (TiLV); Fish cell lines; Susceptibility; Replication
期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.848； 五年影响因子：3.957 ）
ISSN： 0882-4010
年卷期： 2022 年 166 卷
页码：
收录情况： SCI
摘要： Tilapia lake virus disease (TiLVD) caused by Tilapia lake virus (TiLV) is a great threat to the global tilapia culture industry. Effective prevention and control strategies have not been developed due to limited basic research of pathogenesis of TiLVD. Cell lines from different fish species have been found to be permissive to TiLV infection. In the current study, we comprehensively analyzed TiLV susceptibilities to 10 permanent growing fish cell lines. We found that the highest viral titers were generated onto TiB cells originated from the tilapia species Oreochromis mossambicus, MSF from the largemouth bass Micropterus salmoides, CAMK from the hybrid snakehead Channa argus x Channa maculata and SS derived from the perch species Siniperca chuatsi. Viral copy numbers from these four cell lines ranged from 4 x 10(7) copies/mu L to 4.6 x 10(8) copies/mu L. Confocal immunofluorescent microscopy also indicated that all 10 cell lines can support varying degrees of viral infection and replication. TiLV particles can be observed in cells from randomly selected three fish species using electron microscope. This study will assist in research and development of prevention and control of TiLVD.
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