Cytological studies on induced mitogynogenesis in Japanese flounder Paralichthys olivaceus (Temminck et Schlegel)
文献类型： 外文期刊
作者： Hou, Jilun 1 ; Wang, Guixing 1 ; Zhang, Xiaoyan 1 ; Liu, Haijin 2 ;
作者机构： 1.Chinese Acad Fishery Sci, Beidaihe Cent Expt Stn, Qinhuangdao 066100, Peoples R China
2.Chinese Acad Fishery Sci, Ctr Appl Aquat Genom, Beijing 100141, Peoples R China
关键词： Cytology; Hydrostatic pressure; Japanese flounder Paralichthys olivaceus; Mitogynogenesis; Monopolar spindle
期刊名称：ZYGOTE （ 影响因子：1.442； 五年影响因子：1.536 ）
ISSN： 0967-1994
年卷期： 2016 年 24 卷 5 期
页码：
收录情况： SCI
摘要： The effect of hydrostatic pressure treatment on the induction of mitogynogenesis in the eggs of Japanese flounder Paralichthys olivaceus (Temminck et Schlegel) by using heterospecific sperm were studied. Before treatment, the eggs were at metaphase of the first mitosis. The spindle was disassembled by the treatment and then resembled in its pretreatment position, and the chromosomes were rearranged, i.e., the first mitosis was not blocked. During the second mitotic cycle, only a monopolar spindle was assembled in each blastomere and the chromosomes doubled, but cell cleavage was blocked. In the third cycle, mitosis proceeded normally with a bipolar spindle in each blastomere. Flow cytometric analysis of ploidy demonstrated that mitogynogenetic larvae were all diploid. The ultraviolet light-irradiated sperm of the red sea bream (Pagrus major) was condensed, formed a dense chromatin body, and randomly entered one blastomere.
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