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Cit1,2RhaT and two novel CitdGlcTs participate in flavor-related flavonoid metabolism during citrus fruit development

文献类型： 外文期刊

作者： Chen, Jiajing 1 ; Yuan, Ziyu 1 ; Zhang, Haipeng 1 ; Li, Wenyun 1 ; Shi, Meiyan 1 ; Peng, Zhaoxin 1 ; Li, Mingyue 1 ; Tian 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Hort & Forestry, Key Lab Hort Plant Biol, Minist Educ, Wuhan 430070, Hubei, Peoples R China

2.New Zealand Inst Plant & Food Res Ltd, Private Bag 92169, Auckland 1142, New Zealand

3.Guizhou Acad Agr Sci, Guizhou Fruit Inst, Guiyang 550006, Guizhou, Peoples R China

4.Guangdong Acad Agr Sci, Guangdong Fruit Inst, Guangzhou 510640, Guangdong, Peoples R China

关键词： Bitterness; citrus; flavonoid; flavonoid-7-O-di-glucosides; flavonoid-7-O-glucoside; neohesperidoside

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：6.992； 五年影响因子：7.86 ）

ISSN： 0022-0957

年卷期： 2019 年 70 卷 10 期

页码：

收录情况： SCI

摘要： Neohesperidosides are disaccharides that are present in some flavonoids and impart a bitter taste, which can significantly affect the commercial value of citrus fruits. In this study, we identified three flavonoid-7-O-di-glucosyltransferase (dGlcT) genes closely related to 1,2-rhamnosyltransferase (1,2RhaT) in citrus genomes. However, only 1,2RhaT was directly linked to the accumulation of neohesperidoside, as demonstrated by association analysis of 50 accessions and co-segregation analysis of an F-1 population derived from Citrus reticulata x Poncirus trifoliata. In transgenic tobacco BY2 cells, over-expression of CitdGlcTs resulted in flavonoid-7-O-glucosides being catalysed into bitterless flavonoid-7-O-di-glucosides, whereas over-expression of Cit1,2RhaT converted the same substrate into bitter-tasting flavonoid-7-O-neohesperidoside. Unlike 1,2RhaT, during citrus fruit development the dGlcTs showed an opposite expression pattern to CHS and CHI, two genes encoding rate-limiting enzymes of flavonoid biosynthesis. An uncoupled availability of dGlcTs and substrates might result in trace accumulation of flavonoid-7-O-di-glucosides in the fruit of C. maxima (pummelo). Past human selection of the deletion and functional mutation of 1,2RhaT has led step-by-step to the evolution of the flavor-related metabolic network in citrus. Our research provides the basis for potentially improving the taste in citrus fruit through manipulation of the network by knocking-out 1,2RhaT or by enhancing the expression of dGlcT using genetic transformation.

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