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Silencing Taiman impairs larval development in Leptinotarsa decemlineata

文献类型： 外文期刊

作者： Xu, Qing-Yu 1 ; Deng, Pan 1 ; Mu, Li-Li 1 ; Fu, Kai-Yun 2 ; Guo, Wen-Chao 3 ; Li, Guo-Qing 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Educ Minist, Key Lab Integrated Management Crop Dis & Pests, Nanjing 210095, Jiangsu, Peoples R China

2.Xinjiang Acad Agr Sci, Inst Plant Protect, Urumqi 830091, Peoples R China

3.Acad Agr Sci, Inst Microbiol Applicat, Urumqi 830091, Peoples R China

4.Minist Agr, Key Lab Intergraded Management Harmful Crop Vermi, Krishi Bhavan, Peoples R China

关键词： Leptinotarsa decemlineata; Taiman; Development; RNA interference; Lethality

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：3.963； 五年影响因子：4.454 ）

ISSN： 0048-3575

年卷期： 2019 年 160 卷

页码：

收录情况： SCI

摘要： An exploration of novel control strategies for Leptinotarsa decemlineata is becoming more pressing given rapid evolution of insecticide resistance and rise of production loss of potato. Dietary delivery of bacterially expressed double-stranded RNA (dsRNA) is a promising alternative for management. An important first step is to uncover possible RNA-interference (RNAi)-target genes effective against both young and old larvae. Taiman (Tai) is a basic-helix-loop-helix/Per-Arnt-Sim transcription factor that is involved in the mediation of both juvenile hormone (JH) and 20-hydroxyecdysone (20E) signaling. In the present paper, we found that continuous ingestion of dsTai for three days by third (penultimate)-instar larvae caused approximately 20% larval mortality and 80% pupation failure. The larval lethality resulted from failed cuticle and tracheae shedding, which subsequently reduced foliage consumption and nutrient absorption, and depleted lipid stores. In contrast, pupation failure derived from disturbed JH and 20E signals, and disordered nutrient homeostasis including, among others, inhibition of trehalose metabolism and reduction of chitin content. Knockdown of LdTai caused similar larval lethality and pupation impairment in second and fourth (final) larval instars. Therefore, LdTai is among the most attractive candidate genes for RNAi to control L. decemlineata larvae.

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