Comparative transcriptome profiling of immune response against Vibrio harveyi infection in Chinese tongue sole
文献类型： 外文期刊
作者： Xu, Hao 1 ; Xu, Xiwen 1 ; Li, Xihong 1 ; Wang, Lei 1 ; Cheng, Jiayu 4 ; Zhou, Qian 1 ; Chen, Songlin 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Minist Agr, Key Lab Sustainable Dev Marine Fisheries, Yellow Sea Fisheries Res Inst, Qingdao, Shandong, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Shandong, Peoples R China
3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai, Peoples R China
4.Weizhuo Aquaculture Co Ltd, Tangshan Ctr Engn & Technol Flatfish Aquaculture, Tangshan, Peoples R China
期刊名称：SCIENTIFIC DATA （ 影响因子：6.444； 五年影响因子：9.051 ）
ISSN：
年卷期： 2019 年 6 卷
页码：
收录情况： SCI
摘要： Vibrio harveyi is a major bacterial pathogen that causes fatal vibriosis in Chinese tongue sole (Cynoglossus semilaevis), resulting in massive mortality in the farming industry. However, the molecular mechanisms of C. semilaevis response to V. harveyi infection are poorly understood. Here, we performed transcriptomic analysis of C. semilaevis, comparing resistant and susceptible families in response to V. harveyi challenge (CsRC and CsSC) and control conditions (CsRU and CsSU). RNA libraries were constructed using 12 RNA samples isolated from three biological replicates of the four groups. We performed transcriptome sequencing on an Illumina HiSeq platform, and generating a total of 1,095 million paired-end reads, with the number of clean reads per library ranging from 75.27 M to 99.97 M. Through pairwise comparisons among the four groups, we identified 713 genes exhibiting significant differences at the transcript level. Furthermore, the expression levels were validated by real-time qPCR. Our results provide a valuable resource and new insights into the immune response to V. harveyi infection.
- 相关文献
作者其他论文 更多>>
-
Bile acids help maintain immune homeostasis and enhance antioxidant capacity upon Vibrio infection in leopard coral grouper
作者：Zhang, Junwei;Yang, Bowen;Shi, Meng;Chen, Songlin;Zhou, Qian;Zhang, Junwei;Zhang, Jiquan;Wang, Shengpeng
关键词：Plectropomus leopardus; BAs; Vibrio harveyi infection; Transcriptomics; Metabolomics
-
A QTL associated with vibriosis resistance highlights the significant role of jam2a gene in tongue sole (Cynoglossus semilaevis)
作者：Li, Yangzhen;Chi, Yong;Yan, Xu;Hu, Yuanri;Chen, Songlin;Li, Yangzhen;Chen, Songlin;Li, Zhongming;Jia, Lei
关键词：Genome-wide association; Quantitative trait locus; Transcriptome analysis; Heritability; Immune response
-
Orchestrated immune responses to Mycobacterium marinum natural infection in tongue sole ( Cynoglossus semilaevis)
作者：Li, Yangzhen;Yan, Xu;Chi, Yong;Hu, Yuanri;Wang, Jialin;Chen, Songlin;Li, Yangzhen;Chen, Songlin;Song, Limin;Robeldo, Diego;Mukiibi, Robert;Robeldo, Diego;Mukiibi, Robert;Robeldo, Diego;Mukiibi, Robert
关键词：Mycobacteriosis; Bacterial infection; RNA-Seq; Transcriptome analysis; Disease resistance
-
Genome-wide association study and transcriptome analysis provide the SNPs and molecular insights into the visceral nodular disease tolerance in half-smooth tongue sole (Cynoglossus semilaevis)
作者：Chang, Mengyang;Shi, Kunpeng;Xia, Jinqi;Zhang, Ruixue;Li, Yishuai;Zang, Shaoqing;Tan, Suxu;Wang, Wenwen;Sha, Zhenxia;Chen, Songlin;Sha, Zhenxia;Chen, Songlin
关键词：Cynoglossus semilaevis; Visceral nodular disease; GWAS; RNA-seq; Disease tolerance; Multi omics
-
A newly developed 20 K SNP array reveals QTLs for disease resistance to Cryptocaryon irritans in tiger pufferfish (Takifugu rubripes)
作者：Li, Yangzhen;Fraslin, Clemence;Chi, Yong;Hu, Yuanri;Wang, Jialin;Chen, Songlin;Li, Yangzhen;Mukiibi, Robert;Robledo, Diego;Li, Yangzhen;Li, Weidong;Li, Weiguo;Hu, Yuanri;Wang, Jialin;Chen, Songlin;Houston, Ross D.;Robledo, Diego
关键词：SNP array; Heritability; Genomic prediction; GWAS; QTL; Selective breeding
-
SHP-1 interacts with NFκB1 to inhibit its phosphorylation and nuclear translocation to exert its anti-bacterial function
作者：Wang, Ningning;Tan, Suxu;Wang, Muyuan;Liu, Hongning;Han, Sen;Wu, Zhendong;Ma, Jie;Chen, Songlin;Sha, Zhenxia;Wang, Ningning;Chen, Songlin;Chen, Songlin;Sha, Zhenxia
关键词：SHP-1; Immune response; Phosphorylation of NF kappa B1; Nuclear translocation
-
Proteomic analysis to explore potential mechanism underlying pseudomale sperm defect in Cynoglossus semilaevis
作者：Li, Xihong;Li, Lu;Cui, Zhongkai;Li, Ming;Sun, Xuexue;Li, Zhijie;Chen, Zhangfan;Ding, Lanqing;Xu, Wenteng;Li, Xihong;Cui, Zhongkai;Li, Ming;Chen, Zhangfan;Xu, Wenteng;Li, Lu;Xu, Dongdong;Xu, Wenteng
关键词：Cynoglossus semilaevis; Spermatogenesis; Proteomic profile; Chaperone; UPS
