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An Immunochromatographic Lateral Flow Strip Test for the Rapid Detection of Danofloxacin in Milk

文献类型： 外文期刊

作者： Yang, Xingdong 1 ; Wang, Yinbiao 3 ; Yang, Jifei 2 ; Sun, Zhongke 1 ; Yue, Zonghao 1 ; Li, Lili 1 ; He, Le 1 ; Hu, Xiaofe 1 ;

作者机构： 1.Zhoukou Normal Univ, Inst Food & Drug Inspect, Zhoukou 466001, Peoples R China

2.Henan Acad Agr Sci, Key Lab Anim Immunol, Henan Prov Key Lab Anim Immunol, Minist Agr, Zhengzhou 450002, Henan, Peoples R China

3.Xinxiang Med Univ, Sch Publ Hlth, Xinxiang 453003, Henan, Peoples R China

关键词： Danofloxacin; Monoclonal antibody; Colloidal gold; Immunochromatographic lateral flow strip test; Rapid and quantitative test

期刊名称：FOOD ANALYTICAL METHODS （ 影响因子：3.366； 五年影响因子：3.07 ）

ISSN： 1936-9751

年卷期： 2019 年 12 卷 11 期

页码：

收录情况： SCI

摘要： An immunochromatographic lateral flow strip test (ILFST) for the rapid detection of danofloxacin (DAN) in milk samples was developed based on colloidal gold-labeled anti-DAN monoclonal antibody 3E9 and a competitive format. Quantitative detections of DAN-spiked samples using the ILFST gave a half inhibitory concentration (IC50) of 0.513 ng/mL and a limit of detection (LOD) of 0.092 ng/mL. Recoveries of DAN from spiked milk samples were from 80.0 to 95.1% within an assay (intra-assay) and from 87.0 to 89.8% between assays (inter-assay). The coefficients of variation (CV) for intra-assay and inter-assay were 2.09-9.93% and 2.42-12.07%, respectively. These data indicated that the ILFST was highly sensitive and reproducible. Detections of authentic milk samples using ILFST and high-performance liquid chromatography (HPLC) showed no significant differences between the two methods. Hence, the ILFST can be widely used for both qualitative and quantitative detection of DAN residues in milk samples.

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