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Assessing the Utility of Multiplexed Liquid Chromatography-Mass Spectrometry for Gluten Detection in Australian Breakfast Food Products

文献类型： 外文期刊

作者： Li, Haili 1 ; Bose, Utpal 1 ; Stockwell, Sally 1 ; Howitt, Crispin A. 3 ; Colgrave, Michelle 1 ;

作者机构： 1.CSIRO, Agr & Food, 306 Carmody Rd, St Lucia, Qld 4067, Australia

2.Henan Acad Agr Sci, Inst Anim Husb & Vet Sci, Zhengzhou 450000, Henan, Peoples R China

3.CSIRO, Agr & Food, GPO Box 1700, Canberra, ACT 2601, Australia

4.Edith Cowan Univ, Sch Sci, 270 Joondalup Dr, Joondalup, WA 6027, Australia

关键词： Coeliac disease (CD); gluten; peptide markers; liquid chromatography-mass spectrometry (LC-MS)

期刊名称：MOLECULES （ 影响因子：4.411； 五年影响因子：4.587 ）

ISSN：

年卷期： 2019 年 24 卷 20 期

页码：

收录情况： SCI

摘要： Coeliac disease (CD) is an autoimmune disorder triggered by the ingestion of gluten that is associated with gastrointestinal issues, including diarrhea, abdominal pain, and malabsorption. Gluten is a general name for a class of cereal storage proteins of wheat, barley, and rye that are notably resistant to gastrointestinal digestion. After ingestion, immunogenic peptides are subsequently recognized by T cells in the gastrointestinal tract. The only treatment for CD is a life-long gluten-free diet. As such, it is critical to detect gluten in diverse food types, including those where one would not expect to find gluten. The utility of liquid chromatography-mass spectrometry (LC-MS) using cereal-specific peptide markers to detect gluten in heavily processed food types was assessed. A range of breakfast products, including breakfast cereals, breakfast bars, milk-based breakfast drinks, powdered drinks, and a savory spread, were tested. No gluten was detected by LC-MS in the food products labeled gluten-free, yet enzyme-linked immunosorbent assay (ELISA) measurement revealed inconsistencies in barley-containing products. In products containing wheat, rye, barley, and oats as labeled ingredients, gluten proteins were readily detected using discovery proteomics. Panels comprising ten cereal-specific peptide markers were analyzed by targeted proteomics, providing evidence that LC-MS could detect and differentiate gluten in complex matrices, including baked goods and milk-based products.

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