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Construction of complete degradation pathway for nitrobenzene in Escherichia coli

文献类型： 外文期刊

作者： Deng, Yong-Dong 1 ; Wang, Li-Juan 1 ; Zhang, Wen-Hui 1 ; Xu, Jing 1 ; Gao, Jian-Jie 1 ; Wang, Bo 1 ; Fu, Xiao-Yan 1 ; Han, Hong-Juan 1 ; Li, Zhen-Jun 1 ; Wang, Yu 1 ; Tian, Yong-Sheng 1 ; Peng, Ri-He 1 ; Yao, Quan-Hong 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Shanghai Key Lab Agr Genet & Breeding, Biotechnol Res Inst, 2901 Beidi Rd, Shanghai, Peoples R China

2.Minist Agr & Rural Affairs, Key Lab Safety Assessment Enviornm Agr Genet Modif, Shanghai, Peoples R China

关键词： Organic compounds; Environmental pollution; Bioremediation; Synthetic biology; Engineered bacteria; Degradation

期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY （ 影响因子：7.129； 五年影响因子：7.284 ）

ISSN： 0147-6513

年卷期： 2022 年 243 卷

页码：

收录情况： SCI

摘要： Nitrobenzene is widely present in industrial wastewater and soil. Biodegradation has become an ideal method to remediate organic pollutants due to its low cost, high efficiency, and absence of secondary pollution. In the present study, 10 exogenous genes that can completely degrade nitrobenzene were introduced into Escherichia coli, and their successful expression in the strain was verified by fluorescence quantitative polymerase chain reaction and proteomic analysis. The results of the degradation experiment showed that the engineered strain could completely degrade 4 mM nitrobenzene within 8 h. The formation of intermediate metabolites was detected, and the final metabolites entered the E. coli tricarboxylic acid cycle smoothly. This process was discovered by isotope tracing method. Results indicated the integrality of the degradation pathway and the complete degradation of nitrobenzene. Finally, further experiments were conducted in soil to verify its degra-dation ability and showed that the engineered strain could also degrade 1 mM nitrobenzene within 10 h. In this study, engineered bacteria that can completely degrade nitrobenzene have been constructed successfully. The construction of remediation-engineered bacteria by synthetic biology laid the foundation for the industrial application of biological degradation of organic pollutants.

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