An attenuated Vibrio harveyi surface display of envelope protein VP28 to be protective against WSSV and vibriosis as an immunoactivator for Litopenaeus vannamei
文献类型： 外文期刊
作者： Ma, Yue 1 ; Liu, Yabo 1 ; Wu, Yanyan 1 ; Jia, Lei 4 ; Liu, Xiaohong 1 ; Wang, Qiyao 1 ; Zhang, Yuanxing 1 ;
作者机构： 1.East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
2.Shanghai Engn Res Ctr Mariculture Anim Vaccines, Shanghai 200237, Peoples R China
3.Shanghai Collaborat Innovat Ctr Biomfg, Shanghai 200237, Peoples R China
4.Chinese Acad Fishery Sci, Tianjin Bohai Fishery Res Inst, Tianjin 300221, Peoples R China
关键词： Surface display; Attenuated Vibrio harveyi; Envelope protein VP28; WSSV; Vibriosis; Litopenaeus vanname
期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）
ISSN： 1050-4648
年卷期： 2019 年 95 卷
页码：
收录情况： SCI
摘要： Surface display can expose foreign antigenic protein on the surface of the vaccine vector, which is promising choice to elicit better immune responses. In this study, we apply this strategy to develop an immunoactivator by using a live attenuated Vibrio harveyi as an antigenic protein carrier with surface displayed VP28, a major envelope protein of white spot syndrome virus (WSSV), for two major pathogens of Litopenaeus vannamei. As a result, the immunoactivator showed self-limited growth and attenuation of virulence in shrimp via different inoculation routes either with single-repetitive dose or high dose. Moreover, either intramuscular injection or oral administration of the immunoactivator did not affect growth of shrimp body weight or cause pathologic changes. Additionally, the rapid immunoprotection was induced by the immunoactivator after administration for one week with highly relative percent survival (RPS) more than 90% against both V. harveyi and WSSV. Until 4 weeks post administration, the immunoactivator still possessed efficient immune effect with no less than 60% RPS for both pathogens. Totally, the attenuated V. harveyi surface displaying VP28 could be a potential immunoactivator for WSSV and vibriosis control in L. vannamei.
- 相关文献
作者其他论文 更多>>
-
Exploring the key flavor compounds and aroma profiles in traditionally fermented golden pompano (Trachinotus ovatus): Perspective from molecular sensory science
作者：Li, Yujie;Wu, Yanyan;Chen, Shengjun;Zhao, Yongqiang;Li, Chunsheng;Xiang, Huan;Wang, Di;Wang, Yueqi;Li, Yujie;Wu, Yanyan;Chen, Shengjun;Zhao, Yongqiang;Li, Chunsheng;Wang, Yueqi;Li, Yujie
关键词：Fermented golden pompano; Molecular sensory science; Gas chromatography-olfactometry-mass spectrometry; Aroma dilution analysis; Aroma recombination; Omission experiments; Omission experiments
-
A QTL associated with vibriosis resistance highlights the significant role of jam2a gene in tongue sole (Cynoglossus semilaevis)
作者：Li, Yangzhen;Chi, Yong;Yan, Xu;Hu, Yuanri;Chen, Songlin;Li, Yangzhen;Chen, Songlin;Li, Zhongming;Jia, Lei
关键词：Genome-wide association; Quantitative trait locus; Transcriptome analysis; Heritability; Immune response
-
A smartphone sensing fluorescent detection of mercury ion based on silicon quantum dots in environment water
作者：Zhang, Yuanxing;Shi, Yongfu;Yuan, Rui;Ye, Hongli;Chi, Hai;Zhang, Yuanxing;Ren, Dandan;Chi, Hai;Ye, Hongli;Yin, Xue-Bo
关键词：Silicon quantum dots; Detection; Mercury ion; Fluorescence; Smartphone; Environment water
-
Identification of a novel circovirus associated with turbot (Scophthalmus maximus) acute hemorrhage disease
作者：Wang, Huilin;Huang, Zhihui;Li, Jie;Jiang, Jingzhe;Xu, Liming;Zhou, Yong;Qin, Qiwei;Wei, Jingguang;Wang, Qiyao;Xiao, Zhizhong;Li, Jie
关键词：Turbot; Turbot acute hemorrhage disease; Circovirus; Pathogen
-
Unlocking the chemical basis of fermented golden pompano (Trachinotus ovatus) inoculated with indigenous Bacillus subtilis: Focus on the role of lipid oxidation on volatile flavor formation
作者：Wang, Huifang;Wu, Yanyan;Chen, Shengjun;Zhao, Yongqiang;Li, Chunsheng;Xiang, Huan;Wang, Di;Wei, Ya;Wang, Yueqi;Wu, Yanyan;Chen, Shengjun;Zhao, Yongqiang;Wang, Yueqi;Wang, Huifang
关键词：Fermented golden pompano; Bacillus subtilis; Inoculation fermentation; Volatile compound; Lipid oxidation; Metabolic pathway
-
Transposon insertion sequencing analysis reveals conditional essential genes for infection in Pseudomonas plecoglossicida
作者：Ding, Haoyuan;Ahat, Peherden;Zhang, Yibei;Wang, Qiyao;Song, Wei;Zhang, Yuanxing;Zhang, Yibei;Wang, Qiyao;Zhang, Yibei;Wang, Qiyao;Choi, Sang Ho
关键词：Conditional essential genes; Large yellow croaker ( Larimichthys crocea ); MlaE; Pseudomonas plecoglossicida; Transposon insertion sequencing (Tn-seq)
-
Characterization of firmness in fermented sea bass ( Lateolabrax japonicas) by multidimensional integration strategies: Insights from proteomic and microstructural analyses
作者：Chen, Qian;Li, Jun;Chen, Qian;Nie, Shi;Wu, Yanyan;Chen, Shengjun;Zhao, Yongqiang;Xiang, Huan;Wang, Di;Wang, Yueqi;Wu, Yanyan;Chen, Shengjun;Zhao, Yongqiang;Wang, Yueqi;Chen, Qian
关键词：Fermented sea bass; Proteomics; Firmness; Microstructure
