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Construction of a genetic linkage map of Lentinula edodes based on SSR, SRAP and TRAP markers

文献类型： 外文期刊

作者： Dong, Hui 1 ; Shang, Xiaodong 2 ; Zhao, Xiaoyan 1 ; Yu, Hailong 2 ; Jiang, Ning 2 ; Zhang, Meiyan 2 ; Tan, Qi 2 ; Zhou, C 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Inst Agrofood Qual Standatd & Testing Technol, Lab Agrofood Qual & Safety Risk Assessment, Shanghai 201403, Peoples R China

2.Shanghai Acad Agr Sci, Inst Edible Fungi, Natl Res Ctr Edible Fungi Biotechnol & Engn, Shanghai 201403, Peoples R China

关键词： Lentinula edodes; genetic linkage map; SSR

期刊名称：BREEDING SCIENCE （ 影响因子：2.086； 五年影响因子：2.632 ）

ISSN： 1344-7610

年卷期： 2019 年 69 卷 4 期

页码：

收录情况： SCI

摘要： Genetic mapping is a basic tool for eukaryotic genomic research. It allows the localization of genes or quantitative trait loci (QTLs) and map-based cloning. In this study, we constructed a linkage map based on DNA samples from a commercial strain L808, including two parental monokaryons and 93 single spore isolates considered with segregating to 1:1:1:1 at four mating types (A(1)B(1), A(1)B(2), A(2)B(1) and A(2)B(2)). Using Simple Sequence Repeats (SSR), Sequence Related Amplified Polymorphism (SRAP), Target Region Amplified Polymorphism (TRAP) molecular markers, 182 molecular markers and two mating factors were located on 11 linkage groups (LGs). The total length of the map was 948.083 centimorgan (cM), with an average marker interval distance of 4.817 cM. Only two gaps spanning more than 20 cM was observed. The probability of 20 cM, 10 cM, 5 cM genetic distance cover one marker was 99.68%, 94.36%, 76.43% in our genetic linkage map, respectively. This is the first linkage map of Lentinula edodes using SSR markers, which provides essential information for quantitative trait analyses and improvement of genome assembly.

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