Quantification of Enterocytozoon hepatopenaei (EHP) in Penaeid Shrimps from Southeast Asia and Latin America Using TaqMan Probe-Based Quantitative PCR
文献类型： 外文期刊
作者： Piamsomboon, Patharapol 1 ; Choi, Seong-Kyoon 2 ; Hanggono, Bambang 4 ; Nuraini, Yani Lestari 4 ; Wati, Fatma 4 ; T 1 ;
作者机构： 1.Chulalongkorn Univ, Fac Vet Sci, Dept Vet Med, Bangkok 10330, Thailand
2.DGIST, Core Prot Resources Ctr, Daegu 42988, South Korea
3.DGIST, Div Biotechnol, Daegu 42988, South Korea
4.Brackishwater Aquaculture Dev Ctr, Fish Hlth & Environm Lab, Situbondo 68351, Indonesia
5.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
6.Kyungpook Natl Univ, Coll Vet Med, Daegu 41566, South Korea
7.Korea Res Inst Biosci & Biotechnol, Infect Dis Res Ctr, Daejeon 34141, South Korea
关键词： Enterocytozoon hepatopenaei; qPCR; microsporidian; shrimp parasite
期刊名称：PATHOGENS （ 影响因子：3.492； 五年影响因子：4.066 ）
ISSN：
年卷期： 2019 年 8 卷 4 期
页码：
收录情况： SCI
摘要： We developed a qPCR assay based on the beta-tubulin gene sequence for the shrimp microsporidian parasite Enterocytozoon hepatopenaei (EHP). This assay reacted with the hepatopancreas (HP) of EHP-infected shrimps, and the highest copy numbers were found in HP and feces samples from Southeast Asian countries (10(6)-10(8) copies mg(-1)), while HP samples from Latin America, Artemia, and EHP-contaminated water showed lower amounts (10(1)-10(3) copies mg(-1) or mL(-1) of water). No false positive was found with the normal shrimp genome, live feeds, or other parasitic diseases. This tool will facilitate the management of EHP infection in shrimp farms.
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