文献类型： 外文期刊
作者： Wang, Chong 1 ; Liu, Shuang 1 ; Tang, Kathy F. J. 1 ; Zhang, Qingli 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Minist Agr,Funct Lab Marine Fisheries Sci & Food, Key Lab Maricultural Organism Dis Control,Qingdao, Qingdao Natl Lab Marine Sci & Technol,Yellow Sea, Qingdao 266071, Peoples R China
关键词： covert mortality nodavirus (CMNV); natural infection; model organism; zebrafish
期刊名称：JOURNAL OF FISH DISEASES （ 影响因子：2.767； 五年影响因子：2.689 ）
ISSN： 0140-7775
年卷期： 2021 年 44 卷 9 期
页码：
收录情况： SCI
摘要： Covert mortality nodavirus (CMNV), a novel aquatic pathogen, causes viral covert mortality disease (VCMD) in shrimps and also known to infect farmed marine fish. To date, there has no report regarding the ability of this virus to infect freshwater fish. In this study, we screened and discovered CMNV-positive freshwater zebrafish individuals by reverse transcription-nested PCR (RT-nPCR). The sequence of CMNV amplicons from zebrafish was found to share 99% identity with RNA-dependent RNA polymerase (RdRp) gene of the original CMNV isolate. Histopathological examination of the CMNV-positive zebrafish samples revealed extensive vacuolation and karyopyknosis lesions in the retina of the eye and the midbrain mesencephalon. CMNV-like virus particles were visualized in these tissues under transmission electron microscope. Different degrees of pathological damages were also found in muscle, gills, thymus and ovarian tissues. Strong positive signals of CMNV probe were observed in these infected tissues by in situ hybridization. Overall, all results indicated that zebrafish, an acknowledged model organism, could be infected naturally by CMNV. Thus, it is needed to pay close attention to the possible interference of CMNV whether in assessment of toxic substances, or in studying the developmental characterization and the nerval function, when zebrafish was used as model animal.
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