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Development of an immunochromatographic lateral flow strip test for the rapid detection of diclofenac in medicinal wine

文献类型： 外文期刊

作者： Yang, Xingdong 1 ; Wan, Yinbiao 3 ; Yang, Jifei 2 ; Sun, Zhongke 1 ; Chu, Cuiwei 1 ; Yue, Zonghao 1 ; Li, Lili 1 ; Hu, Xi 1 ;

作者机构： 1.Zhoukou Normal Univ, Inst Food & Drug Inspect, Zhoukou, Peoples R China

2.Henan Acad Agr Sci, Key Lab Anim Immunol, Henan Prov Key Lab Anim Immunol, Minist Agr, Zhengzhou 450002, Peoples R China

3.Xinxiang Med Univ, Sch Publ Hlth, Xinxiang, Henan, Peoples R China

关键词： Diclofenac; colloidal gold; lateral flow immunoassay; rapid detection

期刊名称：FOOD AND AGRICULTURAL IMMUNOLOGY （ 影响因子：3.101； 五年影响因子：2.573 ）

ISSN： 0954-0105

年卷期： 2020 年 31 卷 1 期

页码：

收录情况： SCI

摘要： A rapid immunochromatographic lateral flow strip test (ILFST) of competitive format for the detection of diclofenac (DCF) in medicinal wine samples was developed. The half-inhibitory concentration (IC50) values of the ILFST for DCF and the limit of detection (LOD) in DCF-spiked samples were 0.66 and 0.15 ng/mL, respectively. Recoveries of DCF from spiked medicinal wine samples were from 84.4% to 85.9% within an assay (intra-assay) and from 83.7% to 88.4% between assays (inter-assay). The coefficients of variation (CV) for intra-assay and inter-assay were 4.10-7.56% and 4.71-8.94%, respectively. These results indicated that the ILFST was highly sensitive and re-producible. The same authentic medicinal wine samples were tested by both ILFST and high-performance liquid chromatography (HPLC) respectively. The results showed no significant differences between the two methods. Therefore, the ILFST can be widely used as a convenient tool for both qualitative and quantitative detection of DCF residues in medicinal wine samples.

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