The circular RNA aplacirc_13267 upregulates duck granulosa cell apoptosis by the apla-miR-1-13/THBS1 signaling pathway
文献类型： 外文期刊
作者： Wu, Yan 1 ; Xiao, Hongwei 1 ; Pi, Jinsong 1 ; Zhang, Hao 1 ; Pan, Ailuan 1 ; Pu, Yuejin 1 ; Liang, Zhenhua 1 ; Shen, Jie; 1 ;
作者机构： 1.Hubei Acad Agr Sci, Inst Anim Husb & Vet, Wuhan 430064, Peoples R China
2.Inst Anim Husb & Vet, Hubei Key Lab Anim Embryo & Mol Breeding, Wuhan, Peoples R China
关键词： circular RNA; duck; follicles development; granulosa cells
期刊名称：JOURNAL OF CELLULAR PHYSIOLOGY （ 影响因子：6.384； 五年影响因子：5.987 ）
ISSN： 0021-9541
年卷期： 2020 年 235 卷 7-8 期
页码：
收录情况： SCI
摘要： Follicle development is a key factor that determines the reproductive performance of poultry. The existing evidence suggests that circular RNAs (circRNAs) play an important role in a variety of biological processes, especially in posttranscriptional regulation, but the regulatory mechanism of circRNAs in duck follicle development has rarely been reported. To better explore the molecular mechanism of follicle development in ducks, we sequenced and analyzed the follicular circRNAs; 4,204 circRNAs were predicted in the duck follicles. Fourteen circRNAs were differentially expressed between the white follicles and yellow follicles. The results of our studies showed that aplacirc_013267 promoted cell apoptosis in duck GCs. Moreover, a bioinformatics prediction analysis demonstrated that aplacirc_013267 was involved in a circRNA-miRNA-mRNA coexpression network and was observed to sponge two follicle-related miRNAs by a luciferase activity assay. Furthermore, we found that overexpression of aplacirc_013267 significantly increased thrombospondin-1 (THBS1) expression and downregulated granulosa cell apoptosis. The mechanistic study showed that aplacirc_013267 directly binds to and inhibits apla-mir-1-13; then, aplacirc_013267 increases the expression of THBS1 and upregulates granulosa cell apoptosis. Taken together, our findings demonstrate that circRNAs have potential effects in duck ovarian follicles and that circRNAs may represent a new avenue to understand follicular development.
- 相关文献
作者其他论文 更多>>
-
High temperature curing softening salted egg yolk: Insights on microstructure, texture properties and composition of key protein
作者：Sun, Jing;Wu, Yan;Zeng, Qi;Liu, Wenhao;Du, Jinping;Pi, Jinsong;Lu, Lizhi;Zeng, Tao;Harlina, Putri Widyanti;Yang, Fang
关键词：Proteomics; Lipoprotein; Rheological property; Aggregation; Protein oxidation
-
MiR-106a-5p by Targeting MAP3K2 Promotes Repair of Oxidative Stress Damage to the Intestinal Barrier in Prelaying Ducks
作者：Zhang, Li;Luo, Xiang;Tang, Rui;Wu, Yan;Liang, Zhenhua;Pi, Jinsong;Zhang, Hao;Zhang, Li;Luo, Xiang;Tang, Rui;Liu, Jingbo
关键词：apla-miR-106a-5p; intestinal barrier function; oxidative stress; prelaying ducks
-
Deciphering the carbon and nitrogen component conversion in humification process mediated by distinct microbial mechanisms in composting from different domestic organic wastes
作者：Zhan, Yabin;Chen, Yunfeng;Yang, Li;Liu, Bo;Zhan, Yabin;Li, Ruoqi;Chen, Wenjie;Chen, Yunfeng;Yang, Li;Liu, Bo;Li, Jun;Chang, Yuan;Wei, Yuquan;Li, Ji;Zhan, Yabin;Li, Ruoqi;Chen, Wenjie;Tao, Xingling;Li, Jun;Chang, Yuan;Wei, Yuquan;Li, Ji;Chen, Peizhen;Wang, Zhigang;Zhang, Hao
关键词：Food waste; Vegetable waste; Composting; Humic acid; Microbial community
-
Differential analysis of ovarian tissue between high and low-yielded laying hens in the late laying stage and the effect of LECT2 gene on follicular granulosa cells proliferation
作者：Chen, Lin;Li, Xianqiang;Wu, Yan;Pi, Jinsong;Chen, Lin;Li, Xianqiang;Wang, Jiaxiang
关键词：Laying hens; Laying performance; Oxidative stress; RNA-seq; LECT2 gene
-
Quasi-Targeted Metabolomics Approach Reveal the Metabolite Differences of Three Poultry Eggs
作者：Wu, Yan;Xiao, Hongwei;Zhang, Hao;Pan, Ailuan;Shen, Jie;Sun, Jing;Liang, Zhenhua;Pi, Jinsong;Wu, Yan
关键词：chicken egg; duck egg; quail egg; metabolomics; metabolite
-
Integrated transcriptomics and metabolomics analysis provide insight into the resistance response of rice against brown planthopper
作者：Shi, Shaojie;Zha, Wenjun;Yu, Xinying;Wu, Yan;Li, Sanhe;Xu, Huashan;Li, Peide;Li, Changyan;Liu, Kai;Chen, Junxiao;Yang, Guocai;Chen, Zhijun;Wu, Bian;Wan, Bingliang;Zhou, Lei;You, Aiqing;Zhou, Lei;You, Aiqing
关键词：rice; brown planthopper; plant-insect interaction; multi-omics analysis; Bph30
-
Single-Cell RNA sequencing of leaf sheath cells reveals the mechanism of rice resistance to brown planthopper (Nilaparvata lugens)
作者：Zha, Wenjun;Li, Changyan;Wu, Yan;Chen, Junxiao;Li, Sanhe;Wu, Bian;Shi, Shaojie;Xu, Huashan;Li, Peide;Liu, Kai;Yang, Guocai;Chen, Zhijun;Xu, Deze;Zhou, Lei;You, Aiqing;Zha, Wenjun;Xu, Deze;Zhou, Lei;You, Aiqing;Sun, Minshan
关键词：single-cell; RNA-seq; leaf sheath cells; resistant; Nilaparvata lugens; rice
