文献类型： 外文期刊
作者： Tian, Yong-Sheng 1 ; Fu, Xiao-Yan 1 ; Yang, Zun-Qiu 1 ; Wang, Bo 1 ; Gao, Jian-Jie 1 ; Wang, Ming-Qing 2 ; Xu, Jing 1 ; H 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China
2.Shanghai Normal Univ, Coll Life Sci, Shanghai 200234, Peoples R China
关键词： betanin biosynthesis; biofortification; food additives; multigene metabolic engineering; rice endosperm
期刊名称：NEW PHYTOLOGIST （ 影响因子：10.151； 五年影响因子：10.475 ）
ISSN： 0028-646X
年卷期： 2020 年 225 卷 5 期
页码：
收录情况： SCI
摘要： Betanin has been widely used as an additive for many centuries, and its use has increased because of its market application as an additive, high free radical scavenging activity, and safety, health-promoting properties. The main source of betanin is red beet, but many factors notably affect the yield of betanin from red beets. Betanin is not produced in cereal grains. Thus, developing biofortified crops with betanin is another alternative to health-promoting food additives. Here, rice endosperm was bioengineered for betanin biosynthesis by introducing three synthetic genes (meloS, BvDODA1S, and BvCYP76AD1S). The overexpression of these genes driven by rice endosperm-specific promoter established the betanin biosynthetic pathways in the endosperm, resulting in new types of germplasm - 'Betanin Rice' (BR). The BR grains were enriched with betanin and had relatively high antioxidant activity. Our results proved that betanin can be biosynthesized de novo in rice endosperm by introducing three genes in the committed betanin biosynthetic pathway. The betanin-fortified rice in this study can be used as a functional grain to promote health and as a raw material to process dietary supplements.
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