Metabolomics Analysis of the Peels of Different Colored Citrus Fruits (Citrus reticulata cv. 'Shatangju') During the Maturation Period Based on UHPLC-QQQ-MS
文献类型: 外文期刊
作者: Wang, Feiyan 1 ; Huang, Yongjing 2 ; Wu, Wen 2 ; Zhu, Congyi 2 ; Zhang, Ruimin 2 ; Chen, Jiezhong 1 ; Zeng, Jiwu 2 ;
作者机构: 1.South China Agr Univ, Coll Hort, Guangzhou 510642, Peoples R China
2.Guangdong Acad Agr Sci, Inst Fruit Tree Res, Key Lab South Subtrop Fruit Biol & Genet Resource, Key Lab Trop & Subtrop Fruit Tree Res, Guangzhou 510640, Peoples R China
关键词: Citrus reticulata cv; 'Shatangju'; Huanglongbing (HLB); metabolomics; PCA; OPLS-DA; UHPLC-QQQ-MS
期刊名称:MOLECULES ( 2020影响因子:4.411; 五年影响因子:4.587 )
年卷期: 2020 年 25 卷 2 期
收录情况: SCI
摘要: Citrus is a globally consumed fruit with great popularity. Mandarin (Citrus reticulata cv. 'Shatangju') is a local variety, and its planting area and yield are the greatest regarding fruit tree planting in Guangdong Province, China. However, its resistance to Huanglongbing (HLB) is weak. After infection by HLB, the fruits cannot develop normally. In this study, four kinds of fruits were classified as HBG, XQG, ZQG, and DHG, according to the color of their peels. The metabolomes of the three abnormally colored groups (HBG, XQG, and ZQG) and the normally colored group (DHG) were compared using a UPLC-QQQ-MS-based metabolomics approach. In total, 913 metabolites were identified and classified into 23 different categories, including phenylpropanoids and flavonoids; among them, 215 (HBG, 177; XQG, 124; and ZQG, 62) metabolites showed differential accumulation in the three comparison groups (HBG/XQG/ZQG versus DHG). A total of 2 unique metabolites, O-caffeoyl maltotriose and myricetin were detected only in DHG samples. When comparing HBG with DHG, there were 109 decreased and 68 increased metabolites; comparing XQG with DHG, there were 88 decreased and 36 increased metabolites; comparing ZQG with DHG, 41 metabolites were decreased, and 21 metabolites were increased. Metabolic pathway enrichment analysis of these differential metabolites showed significant enrichment of the "phenylpropanoid biosynthesis" pathway in all comparison groups. The hierarchical cluster analysis of the differential metabolites of the four groups showed a clear grouping patterns. The relative contents of three phenylpropanoids, four flavonoids, two alkaloids, one anthocyanin, and two other metabolites were significantly different between each comparison group. This study might provide fundamental insight for the isolation and identification of functional compounds from the peels of citrus fruit infected with HLB and for in-depth research on the effect of HLB on the formation of fruits pigment and the development of HLB-resistant citrus varieties.
- 相关文献
作者其他论文 更多>>
-
Antibacterial Activity of Ethanol Extract from Australian Finger Lime
作者:Zhang, Ruimin;Fan, Zhengyan;Zhu, Congyi;Huang, Yongjing;Wu, Pingzhi;Zeng, Jiwu
关键词:antibacterial effect; Australian finger lime (Citrus australasica L.); ethanol extract; minimum inhibitory concentration; phytochemicals
-
Flavor characterization of Citri Reticulatae Pericarpium (Citrus reticulata 'Chachiensis') with different aging years via sensory and metabolomic approaches
作者:Liu, Yuan;Wen, Huan;Kong, Jiatao;Hu, Zhehui;Zhang, Hongyan;Chen, Jiajing;Xu, Juan;Liu, Yuan;Wen, Huan;Kong, Jiatao;Hu, Zhehui;Zhang, Hongyan;Chen, Jiajing;Xu, Juan;Hu, Yang;Zeng, Jiwu;Chen, Xiangling
关键词:Guangchenpi (GCP); Aging process; Sensory evaluation; Flavor -related metabolites; Characteristic aroma compounds; Predictive modeling
-
Integrated untargeted metabolomic and transcriptomic analyses reveal OMT genes controlling polymethoxyflavonoid biosynthesis in the pericarp of Citrus reticulata 'Chachi'
作者:Li, Xinqi;Mao, Genlin;Chen, Wanbing;Wu, Pingzhi;Zhang, Ruimin;Zhang, Man;Huang, Yongjing;Zeng, Jiwu;Li, Xinqi;Mao, Genlin;Chen, Wanbing;Wu, Pingzhi;Zhang, Ruimin;Zhang, Man;Huang, Yongjing;Zeng, Jiwu;Li, Xinqi;Mao, Genlin;Chen, Wanbing;Wu, Pingzhi;Zhang, Ruimin;Zhang, Man;Huang, Yongjing;Zeng, Jiwu;Li, Xinqi;Xu, Juan;Li, Xinqi;Xu, Juan
关键词:Citrus functional qualities; Polymethoxyflavonoid; Potential biomarkers; Multi-omics combined analysis; Gene expression
-
Bruceine D inhibits the growth of Spodoptera litura by inducing cell apoptosis in the midgut via an oxidative burst
作者:Zeng, Jiwu;Mao, Genlin;Xu, Yuhui;Zeng, Jiwu;Lu, Wei;Xu, Yuhui;Liu, Huan;Xu, Hanhong;Xu, Hanhong
关键词:Bruceine D; Spodoptera litura; growth and development; midgut; ROS; apoptosis
-
Combined analysis of GC-MS, RNA-seq and ATAC-seq elucidates the essential oils variation and terpenes biosynthesis in Citrus grandis 'Tomentosa'
作者:Fan, Ruiyi;Qiu, Diyang;Mao, Genlin;Zeng, Jiwu;Fan, Ruiyi;Qiu, Diyang;Mao, Genlin;Zeng, Jiwu;Fan, Ruiyi;Qiu, Diyang;Mao, Genlin;Zeng, Jiwu
关键词:Essential oils; GC-MS; Transcriptome; ATAC-seq; Terpenes variations; Transcriptional factors
-
A smartphone-based electrochemical sensing platform for the portable and simultaneous determination of flavonoids in Citri Reticulatae Pericarpium
作者:Xia, Hong-qi;Zhu, Congyi;Qiu, Diyang;Zeng, Jiwu
关键词:Portable electrochemical sensing platform; Laser induced graphene; Citri reticulatae pericarpium; Polymethoxylated flavones; Hesperidin
-
The gap-free genome and multi-omics analysis of Citrus reticulata 'Chachi' reveal the dynamics of fruit flavonoid biosynthesis
作者:Zhu, Congyi;Wu, Pingzhi;Huang, Yongjing;Zhang, Ruimin;Fan, Zhengyan;Zeng, Jiwu;You, Congjun;Yu, Chao;Gong, Jinli;Hu, Xiaoli;Sun, Xuepeng;You, Congjun;Yu, Chao;Gong, Jinli;Hu, Xiaoli;Sun, Xuepeng
关键词: