Development and characterization of 26 SNP markers in Ochetobius elongatus based on restriction site-associated DNA sequencing (RAD-seq)
文献类型： 外文期刊
作者： Yang, Jiping 1 ; Li, Yuefei 1 ; Zhu, Shuli 1 ; Chen, Weitao 1 ; Li, Jie 1 ; Xue, Huimin 1 ; Li, Xinhui 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou 510380, Peoples R China
2.Shanghai Ocean Univ, Coll Marine Sci, Shanghai 201306, Peoples R China
关键词： Conservation genetics; Ochetobius elongates; SNP marker; RAD-seq
期刊名称：CONSERVATION GENETICS RESOURCES （ 影响因子：0.973； 五年影响因子：0.946 ）
ISSN： 1877-7252
年卷期： 2020 年 12 卷 1 期
页码：
收录情况： SCI
摘要： Ochetobius elongatus (Cyprinidae, Leuciscinae) was once an endemic species and widely distributed in numbers of rivers throughout South China. Nevertheless, the wild population of O. elongatus reduced dramatically in recent years due to anthropogenic impacts and it has been listed as a critically endangered species by IUCN. In this study, 26 novel single nucleotide polymorphism (SNP) markers were developed based on Restriction-site associated DNA sequencing. The results showed that the minor allele frequency varied from 0.071 to 0.500, the observed heterozygosity and expected heterozygosity ranged from 0.093 to 0.964 and 0.133 to 0.505, respectively. The PIC ranged from 0.124 to 0.375. Six loci showed significant deviations from the HWE after Bonferroni correction (p < 0.05), but no significant linkage disequilibrium was found. These SNP markers will provide valuable information for future population and conservation genetics of this germplasm resource.
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