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Accurate and ultrasensitive detection for PEDV based on photoelectrochemical sensing coupling loop-mediated isothermal amplification

文献类型： 外文期刊

作者： Hong, Honghong 1 ; Yuan, Ruishuang 2 ; Ma, Hanyu 1 ; Xiao, Liting 1 ; Li, Bin 3 ; Wang, Kun 1 ;

作者机构： 1.Jiangsu Univ, Sch Chem & Chem Engn, Zhenjiang 212013, Peoples R China

2.Jiangsu Univ, Sch Agr Engn, Zhenjiang 212013, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing 210014, Peoples R China

4.Qingdao Univ Sci & Technol, Sch Chem & Mol Engn, Key Lab Opt Elect Sensing & Analyt Chem Life Sci, OE, Qingdao 266042, Peoples R China

关键词： Photoelectrochemical sensing platform; Loop-mediated isothermal amplification; Porcine epidemic diarrhea virus

期刊名称：TALANTA （ 影响因子：6.1； 五年影响因子：5.4 ）

ISSN： 0039-9140

年卷期： 2023 年 258 卷

页码：

收录情况： SCI

摘要： Porcine epidemic diarrhea (PED) is a serious disease requiring a simple and accurate detection method. Accordingly, this study developed a novel, ultrasensitive photoelectrochemical (PEC) sensing platform using the loop-mediated isothermal amplification (LAMP) technique (LAMP-PEC). An amino (-NH2)-modified LAMP product is obtained by amplification of the PED virus gene with specially designed primers. The generated NH2- modified LAMP product is assembled on the surface of an electrode by forming imine linkages between aldehyde and amino groups based on the Schiff base reaction. A stable photocurrent is provided by a CdIn2S4 photoactive material, which possesses high photoelectric conversion efficiency. Amplified DNA assembled on the electrode surface increases steric hindrance and hinders electrons from moving from the electrode to electron acceptors, which decreases the photocurrent. This strategy can detect PEDV with a low detection limit of 0.3 fg mu L-1 and a wide linear range of 1 x 10-3-1 x 102 pg/mu L. The sensing platform has excellent specificity and sensitivity and can be used for the quantitative detection of many other pathogens with the assistance of LAMP.

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