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Genetic engineering of complex feed enzymes into barley seed for direct utilization in animal feedstuff

文献类型： 外文期刊

作者： Peng, Ri-He 1 ; Zhang, Wen-Hui 1 ; Wang, Yu 1 ; Deng, Yong-Dong 1 ; Wang, Bo 1 ; Gao, Jian-Jie 1 ; Li, Zhen-Jun 1 ; Wang, Li-Juan 1 ; Fu, Xiao-Yan 1 ; Xu, Jing 1 ; Han, Hong-Juan 1 ; Tian, Yong-Sheng 1 ; Yao, Quan-Hong 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Shanghai Key Lab Agr Genet & Breeding, Biotechnol Res Inst, Shanghai, Peoples R China

2.Minist Agr & Rural Affairs, Key Lab Safety Assessment Environm Agr Genet Modif, Shanghai, Peoples R China

关键词： recombinant enzymes; feed additive; feedstuff; nutrient utilization

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：13.8； 五年影响因子：13.2 ）

ISSN： 1467-7644

年卷期： 2023 年 21 卷 3 期

页码：

收录情况： SCI

摘要： Currently, feed enzymes are primarily obtained through fermentation of fungi, bacteria, and other microorganisms. Although the manufacturing technology for feed enzymes has evolved rapidly, the activities of these enzymes decline during the granulating process and the cost of application has increased over time. An alternative approach is the use of genetically modified plants containing complex feed enzymes for direct utilization in animal feedstuff. We co-expressed three commonly used feed enzymes (phytase, beta-glucanase, and xylanase) in barley seeds using the Agrobacterium-mediated transformation method and generated a new barley germplasm. The results showed that these enzymes were stable and had no effect on the development of the seeds. Supplementation of the basal diet of laying hens with only 8% of enzyme-containing seeds decreased the quantities of indigestible carbohydrates, improved the availability of phosphorus, and reduced the impact of animal production on the environment to an extent similar to directly adding exogenous enzymes to the feed. Feeding enzyme-containing seeds to layers significantly increased the strength of the eggshell and the weight of the eggs by 10.0%-11.3% and 5.6%-7.7% respectively. The intestinal microbiota obtained from layers fed with enzyme-containing seeds was altered compared to controls and was dominated by Alispes and Rikenella. Therefore, the transgenic barley seeds produced in this study can be used as an ideal feedstuff for use in animal feed.

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