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Sensitive detection of African swine fever virus p54 based on in-situ amplification of disposable electrochemical sensor chip

文献类型： 外文期刊

作者： Yuan, Ruishuang 1 ; Wei, Jie 2 ; Geng, Rui 3 ; Li, Bin 4 ; Xiong, Wei 5 ; Fang, Xueen 6 ; Wang, Kun 1 ;

作者机构： 1.Jiangsu Univ, Minist Educ, Sch Agr Engn, Key Lab Modern Agr Equipment & Technol, Zhenjiang 212013, Peoples R China

2.Jiangsu Univ, Sch Chem & Chem Engn, Zhenjiang 212013, Peoples R China

3.Jiangsu Univ, Sch Life Sci, Zhenjiang 212003, Peoples R China

4.Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing 210014, Peoples R China

5.Tech Ctr Anim Plant & Food Inspect & Quarantine Sh, 1208 Minsheng Rd, Pudong Dist 200135, Shanghai, Peoples R China

6.Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China

7.Fudan Univ, Inst Biomed Sci, Shanghai 200433, Peoples R China

8.Jiangsu Univ, Minist Educ, Sch Chem & Chem Engn, Key Lab Modern Agr Equipment & Technol, Zhenjiang 212013, Peoples R China

关键词： Disposable electrochemical sensor chip; Screen printed carbon electrode; Loop-mediated isothermal amplification; African swine fever virus p54; Gold nanoparticles

期刊名称：SENSORS AND ACTUATORS B-CHEMICAL （ 影响因子：8.4； 五年影响因子：7.2 ）

ISSN：

年卷期： 2023 年 380 卷

页码：

收录情况： SCI

摘要： Sensitive detection of African swine fever virus (ASFV) is important to reduce the loss of pig industry. A highly sensitive disposable electrochemical sensor chip for the detection of ASFV p54 was developed by combining insitu loop-mediated isothermal amplification (LAMP) with electrochemical sensing technology. The disposable electrochemical sensor chip was prepared by screen printed carbon electrode (SPCE) covered with polydimethylsiloxane membrane as micro-electrolytic cell, and the primers designed according to the virus were fixed on the gold nanoparticles (Au NPs) modified SPCE. The target gene was amplified by in-situ LAMP within 42 min, and the deposition of Au NPs can promote the electron transfer, enhance the electrochemical signal of [Fe(CN)(6)]3(-/4-) probe and improve the sensitivity. The detection range of the biosensor was 1.0 x 10(-12)-1.0 x 10(-6) g/L, the detection limit was 3.0 x 10(-13) g/L (0.48 copies/mu L). In addition, the sensor has good results for the detection of real samples and has broad application prospects.

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