Strip biosensors based on broad-spectrum aptamers and cationic polymers for the on-site rapid detection of tetracycline antibiotics residues in milk
文献类型： 外文期刊
作者： Zhai, Shengxi 1 ; Bai, Mengyuan 1 ; Yue, Fengling 2 ; Wang, Haifang 3 ; Huang, Jingcheng 1 ; Dong, Haowei 1 ; Yuan, Bei 1 ; Li, Zhengtao 1 ; Zhang, Pengwei 1 ; Zhao, Mingxin 4 ; Guo, Yemin 1 ; Sun, Xia 1 ; Zhao, Wenping 1 ;
作者机构： 1.Shandong Univ Technol, Sch Agr Engn & Food Sci, 266 Xincun Xilu, Zibo 255049, Shandong, Peoples R China
2.Heze Univ, Sch Agr & Bioengn, 2269 Daxue Rd, Heze 274015, Shandong, Peoples R China
3.Beijing Univ Chinese Med, Dongzhimen Hosp, Beijing 100700, Peoples R China
4.Gansu Acad Agr Sci, Inst Fruit & Floriculture, Lanzhou 730070, Peoples R China
关键词： Lateral flow chromatography strip; Broad-spectrum aptamers; Cationic polymer; Antibiotics multi-residue; Food safety
期刊名称：FOOD CHEMISTRY （ 影响因子：9.8； 五年影响因子：9.7 ）
ISSN： 0308-8146
年卷期： 2025 年 464 卷
页码：
收录情况： SCI
摘要： A lateral flow chromatography strip (LFS) is a chromatography-based biosensor with advantages of convenient portability, simple operation and rapid detection. In this study, a novel rapid detection technique of aptamerbased chromatography strip was developed and used for the first time for the residue detection of tetracycline antibiotics (TCs) in various milk samples. In this method, gold nanoparticles (AuNPs) modified by TCs specific aptamers were used as probes, and cationic polymers as capture molecules in a test line (T-line). Meanwhile, the analysis of the gray value of the T-line enabled a linear detection range of 1-300 nM and a limit of detection (LOD) of 0.33 nM for quantitative analysis. The biosensor demonstrated high specificity, good stability, and successfully detected tetracyclines in milk samples with recovery rate of 93.60%-106.20%. This method sets a basis for multi-residue antibiotics detection in diverse samples, showing potential for on-site applications.
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