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Assembling "Balloon-String" Colorimetric Probes through Click Chemistry and Hybridization Chain Reaction for Dual-Signal Amplification in Aptamer-Based Lateral Flow Assays

文献类型： 外文期刊

作者： Dong, Haowei 1 ; Zhang, Pengwei 1 ; Wang, Haifang 2 ; Huang, Jingcheng 1 ; Guo, Zhen 1 ; Cai, Ziping 3 ; Darwish, Ibrahim A. 4 ; Guo, Yemin 1 ; Sun, Xia 1 ;

作者机构： 1.Shandong Univ Technol, Coll Agr Engn & Food Sci, Zibo 255049, Shandong, Peoples R China

2.Beijing Univ Chinese Med, Dongzhimen Hosp, Beijing 100700, Peoples R China

3.Gansu Acad Agr Sci, Inst Chinese Herbal Med, Lanzhou 730070, Gansu, Peoples R China

4.King Saud Univ, Coll Pharm, Dept Pharmaceut Chem, Riyadh 11451, Saudi Arabia

期刊名称：ANALYTICAL CHEMISTRY （ 影响因子：6.7； 五年影响因子：6.6 ）

ISSN： 0003-2700

年卷期： 2025 年 97 卷 19 期

页码：

收录情况： SCI

摘要： As a simple and visually assessable point-of-care testing method, lateral flow assays (LFAs) are widely used for detecting procymidone in vegetable samples. Meanwhile, an increasing number of aptamer-based LFAs are being developed for rapid detection. However, the sensitivity of conventional gold nanoparticles (AuNPs) aptamer-based LFAs is limited, which is a challenge to meet the detection requirements. This study proposed a dual amplification strategy of click chemistry and hybridization chain reaction (HCR) to construct a colorimetric probe to enhance the sensitivity. The nucleic acid nanostructure (HCR-ALK) constructed by HCR was used as a programming template to guide the click chemical reaction to induce the cross-linking of AuNPs-N3 and HCR-ALK to form a uniform and stable '' ballon-string '' colorimetric probe (HCR-AuNPs). This strategy achieved dual-signal amplification. Under optimal conditions, the limit of detection (LOD) of this LFA was 5.4 x 10-2 ng/mL. Compared with the conventional AuNPs aptamer-based LFAs, its LOD had been improved by 13.69-fold, and its color display time had been reduced to one-tenth. In specificity and stability experiments, this LFA showed a satisfactory performance. Moreover, it was successfully applied to the detection of procymidone in vegetable samples, with a recovery rate maintained between 93.44%-104.00%. Thus, this study provides a signal amplification strategy to address the issue of insufficient sensitivity in aptamer-based LFAs. This will offer new insights for the development of aptamer-based LFAs.

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