Electroluminescence aptasensor based on tetrahedral DNA nanostructure with exonuclease-assisted target cycling for detection of acetamiprid
文献类型： 外文期刊
作者： Liu, Jingjing 1 ; Geng, Lingjun 1 ; Wang, Haifang 2 ; Huang, Jingcheng 1 ; Zhou, Shuxian 1 ; Yan, Luting 1 ; Tian, Lei 1 ; Mou, Yaoting 1 ; Zhang, Pengwei 1 ; Zhao, Junhao 1 ; Cai, Ziping 3 ; Zhao, Mingxin 4 ; Sun, Xia 1 ; Vernick, Sefi 5 ; Guo, Yemin 1 ; Darwish, Ibrahim A. 6 ;
作者机构： 1.Shandong Univ Technol, Coll Agr Engn & Food Sci, 266 Xincun Xilu, Zibo 255049, Shandong, Peoples R China
2.Beijing Univ Chinese Med, Dongzhimen Hosp, Beijing 100700, Peoples R China
3.Gansu Acad Agr Sci, Inst Chinese Herbal Med, Lanzhou 730070, Gansu, Peoples R China
4.Gansu Acad Agr Sci, Inst Fruit & Floriculture, Lanzhou 730070, Gansu, Peoples R China
5.Volcani Inst ARO, Inst Agr Engn, Dept Sensing Informat & Mechanizat Engn, IL-5025001 Rishon Leziyyon, Israel
6.King Saud Univ, Coll Pharm, Dept Pharmaceut Chem, POB 2457, Riyadh 11451, Saudi Arabia
关键词： Target cycling; Metal-organic framework; Exonuclease; Tetrahedral DNA nanostructure; Aptasensor; Acetamiprid
期刊名称：FOOD RESEARCH INTERNATIONAL （ 影响因子：8.0； 五年影响因子：8.5 ）
ISSN： 0963-9969
年卷期： 2024 年 198 卷
页码：
收录情况： SCI
摘要： In this study, an electroluminescent (ECL) aptasensor that could efficiently and sensitively detect acetamiprid (ACE) in vegetables was constructed based on an exonuclease-assisted target cycling amplification strategy. Bimetallic RuZn-based metal-organic framework (RuZn-MOF), nucleic acid exonuclease VII (Exo VII) and tetrahedral DNA nanostructure (TDN) were used as constituent materials. First, RuZn-MOF was a substrate material with good luminescence performance and was synthesized by a hydrothermal method. Second, TDN was autonomously assembled by an adapted single-step annealing way. In this experiment, an aptamer labeled with ferrocene (Fc) at one end was firstly bound to the tip of TDN. In the presence of the target pesticide of ACE, the stronger affinity of ACE for the aptamer made the aptamer and ACE bind preferentially. At the same time, Exo VII was activated to cleave the single-stranded aptamer, resulting in ACE release and Fc detachment. As a result, Fc moved away from the electrode surface, allowing the ECL signal intensity to be restored. The limit of detection (LOD) (33.33 fg/mL) of this method was lower than those of many reported aptasensors. This strategy provides an uncomplicated and responsive technique for the detection of ACE and offers further development possibilities for detection and analysis of other biomolecules.
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