Prussian blue nanoparticles-based signal amplification in aptamer-based lateral flow assay for procymidone detection
文献类型： 外文期刊
作者： Dong, Haowei 1 ; Huang, Jingcheng 1 ; Guo, Zhen 1 ; Wang, Haifang 2 ; Geng, Lingjun 1 ; Cai, Ziping 3 ; Darwish, Ibrahim A. 4 ; Guo, Yemin 1 ; Sun, Xia 1 ; Wu, Yanfang 5 ;
作者机构： 1.Shandong Univ Technol, Coll Agr Engn & Food Sci, 266 Xincun Xilu, Zibo 255049, Shandong, Peoples R China
2.China Acad Chinese Med Sci, Wangjing Hosp, Beijing 100102, Peoples R China
3.Gansu Acad Agr Sci, Inst Chinese Herbal Med, Lanzhou 730070, Gansu, Peoples R China
4.King Saud Univ, Coll Pharm, Dept Pharmaceut Chem, POB 2457, Riyadh 11451, Saudi Arabia
5.Univ Otago, Dept Chem, Dunedin 9016, New Zealand
关键词： Procymidone; Aptamer; Prussian blue nanoparticles; Lateral flow assay; Rapid detection
期刊名称：JOURNAL OF FOOD COMPOSITION AND ANALYSIS （ 影响因子：4.6； 五年影响因子：4.6 ）
ISSN： 0889-1575
年卷期： 2025 年 144 卷
页码：
收录情况： SCI
摘要： Procymidone is frequently detected in vegetables, prompting stringent regulatory limits in many countries and regions. Despite this, the development of rapid and sensitive detection methods remains limited. In this study, an aptamer-based lateral flow assay (LFA) was developed for procymidone detection, utilizing Prussian blue nanoparticles (PBNPs) as amplified signal markers, chosen for their intrinsic color and enzyme-like properties. An aptamer was coupled with the PBNPs to form a signal probe, enabling the construction of a competitive LFA. Under optimized conditions, the LFA achieved a limit of detection (LOD) of 0.35 ng/mL and a visual limit of detection (vLOD) of 0.64 ng/mL. The enzyme-mimetic activity of PBNPs was harnessed and such a catalytic reaction strategy further improved the assay sensitivity, leading to a further improvement of the LOD by 14.3fold. The assay demonstrated high sensitivity, selectivity, and reproducibility, performing effectively in typical vegetable samples. This study provides a rapid, sensitive and reliable tool for procymidone detection, offering valuable insights and a new strategy for monitoring pesticides.
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