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Establishment and characterization of cell line from fin of Chinense bahaba (Bahaba taipingensis) and transcriptome analysis of its response to megalocytivirus SKIV-SD strain infection

文献类型： 外文期刊

作者： Gong, Zhihong 1 ; Zhang, Qi 1 ; Wang, Na 1 ; Wang, Lei 1 ; Chen, Zhangfan 1 ; Yan, Lin 4 ; Yan, Kuoqiu 4 ; Hu, Guobin 2 ; Chen, Songlin 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, State Key Lab Mariculture Biobreeding & Sustainabl, Qingdao 266071, Shandong, Peoples R China

2.Ocean Univ China, Coll Marine Life Sci, Qingdao 266100, Peoples R China

3.Zhejiang Ocean Univ, Fisheries Coll, Zhoushan 316022, Peoples R China

4.Guangdong Beluga Whale Marine Biotechnol Co Ltd, Huizhou 516300, Peoples R China

关键词： Chinese bahaba; Fin; Cell line; BTF; Megalocytivirus

期刊名称：AQUACULTURE （ 影响因子：3.9； 五年影响因子：4.1 ）

ISSN： 0044-8486

年卷期： 2024 年 592 卷

页码：

收录情况： SCI

摘要： The Chinese bahaba (Bahaba taipingensis) is an endangered fish species with high economic value, found in the southeastern sea of China. In this study, we established the first Chinese bahaba cell line, named BTF, derived from fin tissue. The primary cells were cultured for over 55 passages in L-15 medium with 15% FBS at 27 degrees C. These cells exhibited a fibroblast-like morphology with multipolar shapes. Karyotype testing revealed a chromosome number of 2n = 48. After thawing the cells that had been stored in liquid nitrogen for 10 months, their viability was >80%. The cell was separately transfected with EGFP-N3 plasmid and cy3 labeled scramble siRNA and clear fluorescence were observed. Moreover, the cells displayed sensitivity to megalocytivirus SKIV-SD strain, demonstrating a pronounced cytopathic effect (CPE) 24 h after infection. Transcriptome sequencing analysis of SKIV-SD infected cells revealed highly expressed genes significantly enriched in nucleic acid replication and protein translation-related pathways, such as Ribosome biogenesis, RNA polymerase, Nucleocytoplasmic transport, and DNA replication. In addition, the infected cells showed differential expression of immune-related genes such as cxcr4 and dr5, which are involved in virus infection. In conclusion, the BTF cell line, derived from the fin tissue of the Chinese bahaba, exhibits stable growth and can be stored in liquid nitrogen for extended periods. This cell line plays a crucial role in conserving the germplasm resources of Chinese bahaba and will serve as a valuable reference for establishing cell lines from other tissues of the Chinese bahaba. Furthermore, our findings suggest that this cell line holds significant potential for virology research, foreign protein expression, and in vitro biological studies of Chinese bahaba.

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