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Evaluation of Tembusu virus single-round infectious particle as vaccine vector in chickens

文献类型： 外文期刊

作者： Huang, Yunzhen 1 ; Liu, Zhe 5 ; Zhang, Junqin 2 ; Dong, Jiawen 2 ; Li, Linlin 2 ; Xiang, Yong 2 ; Kuang, Ruihuan 2 ; Gao, Shimin 5 ; Sun, Minhua 2 ; Liu, Yongjie 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Vet Med, Int Joint Lab Anim Hlth & Food Safety, Nanjing, Jiangsu, Peoples R China

2.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangzhou 510640, Peoples R China

3.Minist Agr & Rural Affairs, Key Lab Prevent & Control Avian Influenza & Other, Guangzhou, Peoples R China

4.Key Lab Livestock Dis Prevent & Treatment Guangdon, Guangzhou, Peoples R China

5.Shanxi Agr Univ, Coll Vet Med, Taigu, Peoples R China

关键词： Single-round infectious particle; Tembusu virus; Vaccine; Fowl adenovirus 4; Immunogenicity

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：2.7； 五年影响因子：2.9 ）

ISSN： 0378-1135

年卷期： 2024 年 298 卷

页码：

收录情况： SCI

摘要： Orthoflaviviruses are single-stranded RNA viruses characterized by highly efficient self-amplification of RNA in host cells, which makes them attractive vehicles for vaccines. Numerous preclinical and clinical studies have demonstrated the efficacy and safety of orthoflavivirus replicon vectors for vaccine development. In this study, we constructed Tembusu virus (TMUV) replicon-based single-round infectious particles (SRIPs) as vaccine development platform. To evaluate the potential of TMUV SRIPs as vaccines, we generated SRIPs that express the heterologous Fowl adenovirus 4 (FAdV-4) fiber2 protein and fiber2 head domain, named TMUVRP-fiber2 and TMUVRP-fiber2H, respectively. To assess the immunogenicity of the TMUV SRIPs, SPF chicks were intramuscularly inoculated twice. Our results showed that the TMUVRP-fiber2 vaccines elicited high levels of neutralizing antibodies. Challenge experiments showed that TMUVRP-fiber2 provided full protection against virulent FAdV-4 and significantly reduced viral shedding. Moreover, the immunogenicity of TMUVRP-fiber2H was significantly lower than that of TMUVRP-fiber2, which was reflected in the neutralizing antibody titer, survival rate, and virus shedding after challenge. Therefore, our results suggested that TMUV SRIPs are a promising novel platform for the development of vaccines for existing and emerging poultry diseases.

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