文献类型： 外文期刊

作者： Huang, Tianqing ¹ ; Gu, Wei ¹ ; Liu, Enhui ¹ ; Wang, Bingqian ¹ ; Wang, Gaochao ¹ ; Dong, Fulin ¹ ; Guo, Fuyuan ² ; Jiao, Wenlong ³ ; Sun, Yanchun ¹ ; Wang, Xiance ⁴ ; Li, Shanwei ⁵ ; Xu, Gefeng ¹ ;

作者机构： 1.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Cold Water Fish Ind Technol Innovat Strateg Allian, Harbin, Peoples R China

2.Yantai Jinghai Marine Fishery Co Ltd, Yantai, Peoples R China

3.Gansu Fisheries Res Inst, Lanzhou, Peoples R China

4.Hangzhou Qiandaohu Xun Long Sci tech CO LTD, Hangzhou, Peoples R China

5.Shanghai Ocean Univ, Coll Food Sci & Technol, Dept Food Sci & Engn, Shanghai, Peoples R China

6.Heilongjiang River Fisheries Res Inst, HeSong St 232, Harbin 150070, Peoples R China

关键词： High temperature stress; miR-301b-5p; nfatc2ip; Inflammatory response; NF-?B; MAPK

期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY （ 影响因子：7.129； 五年影响因子：7.284 ）

ISSN： 0147-6513

年卷期： 2022 年 242 卷

页码：

收录情况： SCI

摘要： Rainbow trout (Oncorhynchus mykiss) is a typical cold-water aquaculture fish and a high-end aquatic product. When water temperature exceeds its optimal range of 12-18 C, the immune system of rainbow trout becomes weakened and unbalanced. High temperature in summer and global warming severely impact rainbow trout industry. The focus of this study was to explore the mechanisms regulating the immune response of rainbow trout under high temperature stress and identify molecular elements that account for resistance to high temperature. In this study, individual fish were screened in a high temperature stress experiment and divided into resistant (R) and sensitive (S) groups. The hepatic transcriptome sequencing and analysis of mRNAs and microRNAs of the R, S, and control groups showed that the number of the differentially expressed genes (DEGs) in the S group (9259) was higher than that in the R group (5313). Furthermore, the 1233 genes differentially expressed between S and R groups were mainly enriched in immune-related pathways, including cytokine-cytokine receptor interaction, TNF signaling and IL-17 signaling. Among these DEGs were miR-301b-5p and its target gene that encodes nuclear factor of activated T cells two interacting protein (nfatc2ip). The dual-luciferase reporter system and immuno-fluorescence experiments verified the relationship between miR-301b-5p and nfatc2ip. We also showed that expression levels of miR-301b-5p and nfatc2ip significantly negatively correlated in the liver of rainbow trout under high temperature stress. By performing functional experiments, we showed that activation of miR-301b-5p expression or inhibition of nfatc2ip expression stimulated the phosphorylation of p65, p38, and JNK in the classical nuclear factor kappa-B and mitogen-activated protein kinase pathways under high temperature stress. These manipulations initially promoted the secretion of the pro-inflammatory factor IL-1 beta and then increased the levels of IL-6, IL-12, and TNF-alpha. In addition, activation of miR-301b-5p expression or inhibition of nfatc2ip expression stimulated the repair of the hepatic ultrastructural damage caused by high temperature stress by activating the inflammatory response in rainbow trout liver.