文献类型： 外文期刊
作者： Zhu, Mengting 1 ; Zhang, Hongmei 3 ; Yang, Hua 1 ; Zhao, Zongsheng 2 ; Blair, Hugh T. 4 ; Liang, Huihui 2 ; Wu, Pei 2 ; Yu, Qian 1 ;
作者机构： 1.Xinjiang Acad Agr & Reclamat Sci, State Key Lab Sheep Genet Improvement & Hlth Prod, Shihezi, Peoples R China
2.Shihezi Univ, Coll Anim Sci & Technol, Shihezi, Xinjiang, Peoples R China
3.Shihezi Univ, Sch Med Coll, Affiliated Hosp 1, Shihezi, Xinjiang, Peoples R China
4.Massey Univ, Inst Vet Anim & Biomed Sci, Palmerston North, New Zealand
关键词： Kazakh sheep; Seasonal estrus; GNAQ; Hypothalamic nerve cells; GnRH
期刊名称：THERIOGENOLOGY （ 影响因子：2.923； 五年影响因子：2.843 ）
ISSN： 0093-691X
年卷期： 2022 年 181 卷
页码：
收录情况： SCI
摘要： Kazakh sheep are typical seasonal estrus animals. Their reproductive system regulation mainly involves the complex regulation of the hypothalamic-pituitary-gonadal axis (HPGA), which is also closely related to reproductive hormone secretion. Gonadotropin-releasing hormone (GnRH), synthesized and secreted by the hypothalamus, is the key to controlling sheep reproductive activity. We studied how GNAQ (G protein subunit alpha q) regulates estrus in sheep by controlling GnRH expression and secretion. We used hypothalamic nerve cells as the research model. GNAQ overexpression and RNA interference vectors were constructed and transfected into the hypothalamic nerve cells of fetal Kazakh sheep. qPCR, western blotting, and enzyme-linked immunosorbent assay were used to detect GNAQ gene expression in Kazakh ewe tissues and analyze its regulatory effect on GnRH expression in the hypothalamic nerve cells. The fetal sheep hypothalamic nerve cells were successfully isolated and cultured. qPCR and cell immunofluorescence showed that the purity of positive cells was >95%. The tissue expression profile showed that there were different degrees of GNAQ gene expression in the Kazakh ewe tissue. Expression levels were relatively higher in the hypothalamus, pituitary, brain, and uterine tissues. When GNAQ expression was downregulated in the hypothalamic nerve cells, the upstream genes KISS1 (kisspeptin), GPR54 (KISS1 receptor), and ER (estrogen receptor) were all upregulated, as were the downstream genes PLCB1 (phospholipase C beta 1), PRKCB (protein kinase C beta), and GNRH. At the same time, GnRH secretion levels were also upregulated. GNAQ regulated its downstream gene PLCB1 in the hypothalamic nerve cells, and directly regulated GnRH expression and secretion through the calcium and PRKC signaling pathways. GNAQ also regulated kisspeptin expression, subsequently regulating GnRH expression and secretion indirectly through the kisspeptin-GPR54 signaling pathway. Our results are of great importance for improving the reproductive performance of seasonal-estrus sheep. (c) 2022 Elsevier Inc. All rights reserved.
- 相关文献
作者其他论文 更多>>
-
Design of label-free electrochemical aptasensors for enrofloxacin detection in mutton
作者：Yu, Qian;Yang, Yonglin
关键词：Aptasensor; Enrofloxacin; Impedimetric; Label-free; Mutton; Nanocomposite
-
Genome-wide comparative analysis reveals selection signatures for reproduction traits in prolific Suffolk sheep
作者：Yang, Hua;Zhu, Mengting;Wang, Mingyuan;Zhou, Huaqian;Zheng, Jingjing;Qiu, Lixia;Fan, Wenhua;Yang, Jinghui;Yu, Qian;Yang, Yonglin;Zhang, Wenzhe;Zhu, Mengting;Wang, Mingyuan;Zhou, Huaqian;Zheng, Jingjing;Fan, Wenhua
关键词：selection signatures; fst; reproduction traits; candidate genes; sheep
-
Whole-genome resequencing of the native sheep provides insights into the microevolution and identifies genes associated with reproduction traits
作者：Zhu, Mengting;Yang, Yonglin;Yang, Hua;Wang, Mingyuan;Yu, Qian;Zhu, Mengting;Zhao, Zongsheng;Zheng, Wei;Wang, Mingyuan;Fang, Chenhui;Zhou, Huaqian;Qi, Hangdong;Zhang, Hongmei;Blair, Hugh T.
关键词：Microevolution; Selection signature; Whole-genome resequencing; GWAS; Reproductive trait; Sheep
-
Fabrication of an electrochemical sensor for the rapid and sensitive detection of enrofloxacin in meat samples
作者：Yu, Qian;Yang, Hua;Yang, Yonglin
关键词：Enrofloxacin; Electrochemical Detection; Cobalt-based Hybrid Modified Carbon-Based Electrode; Food Safety; Antibiotics detection
-
Uncovering the candidate genes related to sheep body weight using multi-trait genome-wide association analysis
作者：Li, Yunna;Guo, Jing;Guo, Yuanyuan;Zhang, Chaoxin;Wang, Zhipeng;Li, Yunna;Yang, Hua;Guo, Jing;Yang, Yonglin;Yu, Qian;Guo, Yuanyuan;Zhang, Chaoxin;Wang, Zhipeng;Zuo, Peng;Zuo, Peng
关键词：sheep; body weight; genome-wide association analysis; multi-trait; single-trait
-
Whole-Genome Resequencing of Worldwide Wild and Domestic Sheep Elucidates Genetic Diversity, Introgression, and Agronomically Important Loci
作者：Lv, Feng-Hua;Luo, Ling-Yun;Lu, Ran;Chen, Jian-Fei;Wang, Jian-Kui;Deng, Xue-Mei;Dehghani-Qanatqestani, Mostafa;Li, Meng-Hua;Cao, Yin-Hong;Salehian-Dehkordi, Hosein;Cao, Yin-Hong;Salehian-Dehkordi, Hosein;Liu, Guang-Jian;Liu, Ming-Jun;Li, Wen-Rong;Zhou, Ping;Wang, Xin-Hua;Shen, Min;Gao, Lei;Yang, Jing-Quan;Yang, Hua;Yang, Yong-Lin;Liu, Chang-Bin;Wan, Peng-Cheng;Zhang, Yun-Sheng;Pi, Wen-Hui;Zhou, Ping;Wang, Xin-Hua;Shen, Min;Gao, Lei;Yang, Jing-Quan;Yang, Hua;Yang, Yong-Lin;Liu, Chang-Bin;Wan, Peng-Cheng;Zhang, Yun-Sheng;Pi, Wen-Hui;Ren, Yan-Ling;Shen, Zhi-Qiang;Wang, Feng;Wang, Yu-Tao;Li, Jin-Quan;Hehua, Eer;Liu, Yong-Gang;Esmailizadeh, Ali;Dehghani-Qanatqestani, Mostafa;Charati, Hadi;Rushdi, Hossam E.;Nosrati, Maryam;Stepanek, Ondrej;Olsaker, Ingrid;Curik, Ino;Gorkhali, Neena A.;Paiva, Samuel R.;Caetano, Alexandre R.;Ciani, Elena;Amills, Marcel;Amills, Marcel;Weimann, Christina;Erhardt, Georg;Amane, Agraw;Amane, Agraw;Hanotte, Olivier;Mwacharo, Joram M.;Mwacharo, Joram M.;Mwacharo, Joram M.;Han, Jian-Lin;Han, Jian-Lin;Hanotte, Olivier;Periasamy, Kathiravan;Johansson, Anna M.;Hallsson, Jon H.;Kantanen, Juha;Coltman, David W.;Bruford, Michael W.;Bruford, Michael W.;Lenstra, Johannes A.
关键词：whole-genome sequences; genetic diversity; adaptive introgression; genetic selection; agronomic traits; migration
-
Whole-Genome Resequencing of Worldwide Wild and Domestic Sheep Elucidates Genetic Diversity, Introgression, and Agronomically Important Loci
作者：Lv, Feng-Hua;Luo, Ling-Yun;Lu, Ran;Chen, Jian-Fei;Wang, Jian-Kui;Deng, Xue-Mei;Dehghani-Qanatqestani, Mostafa;Li, Meng-Hua;Cao, Yin-Hong;Salehian-Dehkordi, Hosein;Cao, Yin-Hong;Salehian-Dehkordi, Hosein;Liu, Guang-Jian;Liu, Ming-Jun;Li, Wen-Rong;Zhou, Ping;Wang, Xin-Hua;Shen, Min;Gao, Lei;Yang, Jing-Quan;Yang, Hua;Yang, Yong-Lin;Liu, Chang-Bin;Wan, Peng-Cheng;Zhang, Yun-Sheng;Pi, Wen-Hui;Zhou, Ping;Wang, Xin-Hua;Shen, Min;Gao, Lei;Yang, Jing-Quan;Yang, Hua;Yang, Yong-Lin;Liu, Chang-Bin;Wan, Peng-Cheng;Zhang, Yun-Sheng;Pi, Wen-Hui;Ren, Yan-Ling;Shen, Zhi-Qiang;Wang, Feng;Wang, Yu-Tao;Li, Jin-Quan;Hehua, Eer;Liu, Yong-Gang;Esmailizadeh, Ali;Dehghani-Qanatqestani, Mostafa;Charati, Hadi;Rushdi, Hossam E.;Nosrati, Maryam;Stepanek, Ondrej;Olsaker, Ingrid;Curik, Ino;Gorkhali, Neena A.;Paiva, Samuel R.;Caetano, Alexandre R.;Ciani, Elena;Amills, Marcel;Amills, Marcel;Weimann, Christina;Erhardt, Georg;Amane, Agraw;Amane, Agraw;Hanotte, Olivier;Mwacharo, Joram M.;Mwacharo, Joram M.;Mwacharo, Joram M.;Han, Jian-Lin;Han, Jian-Lin;Hanotte, Olivier;Periasamy, Kathiravan;Johansson, Anna M.;Hallsson, Jon H.;Kantanen, Juha;Coltman, David W.;Bruford, Michael W.;Bruford, Michael W.;Lenstra, Johannes A.
关键词：whole-genome sequences; genetic diversity; adaptive introgression; genetic selection; agronomic traits; migration
