您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Locking-DNA network regulated CRISPR-Cas12a fluorescent aptasensor based on hollow flower-like magnetic MoS2 microspheres for sensitive detection of sulfadimethoxine

文献类型： 外文期刊

作者： Lv, Yan 1 ; Sun, Yuhan 1 ; Khan, Imran Mahmood 1 ; Li, Qiaofeng 1 ; Zhou, You 6 ; Yue, Lin 1 ; Zhang, Yin 3 ; Wang, Zhouping 1 ;

作者机构： 1.Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China

2.Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Peoples R China

3.Chengdu Univ, Key Lab Meat Proc Sichuan, Chengdu 610106, Peoples R China

4.Jiangnan Univ, Natl Engn Res Ctr Funct Food, Wuxi 214122, Peoples R China

5.Jiangnan Univ, Collaborat Innovat Ctr Food Safety & Qual Control, Wuxi 214122, Peoples R China

6.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, Nanjing 210014, Peoples R China

关键词： Flower-like Fe 3 O 4 @MoS 2 microsphere; Locking-DNA network; CRISPR-Cas12a; Aptasensor; Sulfadimethoxine

期刊名称：CHEMICAL ENGINEERING JOURNAL （ 影响因子：15.1； 五年影响因子：14.3 ）

ISSN： 1385-8947

年卷期： 2023 年 459 卷

页码：

收录情况： SCI

摘要： Sulfadimethoxine (SDM) poses a potential threat to the food safety and human health due to its overuse in aquatic or animal husbandry. Therefore, a rapid and sensitive identification method is urgently needed to monitor SDM in food and the environment. Herein, a "turn-on" fluorescence aptasensor based on entropy-driven catalysis (EDC) and CRISPR-Cas12a was proposed for the sensitive determination of SDM. Particularly, to overcome the barriers of coupling the EDC circuit to CRISPR-Cas12a, a novel EDC circuit with a Y-shaped substrate scaffold was designed to lock the activation sequence of CRISPR-Cas12a. Multifunctional hollow flower-like microsphere H-Fe3O4@MoS2 was synthesized as a fluorescence resonance energy transfer (FRET) energy acceptor for ssDNA-FAM to quenching its fluorescence. In the presence of the SDM, activated Cas12acrRNA-A rapidly cleaved the ssDNA-FAM adsorbed on the MoS2 nanosheet to restore the fluorescence signal in the reporter system, which could be utilized to determine the concentration of SDM. Due to the prominent quenching properties and rapid magnetic separation of H-Fe3O4@MoS2 along with the double signal amplification of locking-EDC-CRISPR-Cas12a, the developed aptasensor exhibited the appealing analytical performance with a log-linear range of 0.005-100 ng mL-1 and a limit of detection of 2.86 pg mL-1. Furthermore, the practicability and credibility of the locking-EDC-Cas12a sensoring strategy were validated by spiked recovery experiments of SDM in real samples. Hence, the current strategy provides a promising candidate analytical tool for food and environmental safety.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台