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High-affinity truncated aptamers for detection of Cronobacter spp with magnetic separation-assisted DNAzyme-driven 3D DNA walker

文献类型： 外文期刊

作者： Yang, Ningru 1 ; Ding, Ning 1 ; Qi, Shuo 1 ; Shang, Zixuan 1 ; Ma, Pengfei 1 ; Khan, Imran Mahmood 1 ; Wang, Zhouping 1 ; Xia, Yu 1 ; Zhang, Yin 3 ; Zhang, Lili 4 ;

作者机构： 1.Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Resources, Int Joint Lab Food Safety, Wuxi 214122, Peoples R China

2.Jimei Univ, Jimmie Univ, Coll Ocean Food & Biol Engn, Xiamen 361021, Fujian, Peoples R China

3.Chengdu Univ, Key Lab Meat Proc Sichuan, Chengdu 610106, Peoples R China

4.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, Key Lab Food Qual & Safety Jiangsu Prov, State Key Lab Breeding Base, Nanjing 210014, Peoples R China

5.Jiangnan Univ, Collaborat Innovat Ctr Food Safety & Qual Control, Natl Engn Res Ctr Funct Food, Wuxi 214122, Peoples R China

关键词： Cronobacter spp.; Aptamer; Truncation; DNA walker; Au nanoparticles; Fluorescence detection

期刊名称：MICROCHIMICA ACTA （ 影响因子：5.7； 五年影响因子：5.4 ）

ISSN： 0026-3672

年卷期： 2024 年 191 卷 3 期

页码：

收录情况： SCI

摘要： After optimizing the original aptamer sequence by truncation strategy, a magnetic separation-assisted DNAzyme-driven 3D DNA walker fluorescent aptasensor was developed for detecting the food-borne pathogen Cronobacter species. Iron oxide magnetic nanoparticles (MNPs) modified with a hybrid of truncated aptamer probe and DNAzyme strand (AP-E1) denoted as MNPs@AP-E1, were employed as capture probes. Simultaneously, a DNAzyme-driven 3D-DNA walker was utilized as the signal amplification element. The substrate strand (Sub) was conjugated with the gold nanoparticles (AuNPs), resulting in the formation of AuNPs@Sub, which served as a 3D walking track. In the presence of the target bacteria and Mg2+, E1-DNAzyme was activated and moved along AuNPs@Sub, continuously releasing the signal probe. Under optimized conditions, a strong linear correlation was observed for Cronobacter sakazakii (C. sakazakii) in the concentration range 10(1) to 10(6) CFU mL(-1), with a low detection limit of 2 CFU mL(-1). The fluorescence signal responses for different Cronobacter species exhibited insignificant differences, with a relative standard deviation of 3.6%. Moreover, the aptasensor was successfully applied to determine C. sakazakii in real samples with recoveries of 92.86%-108.33%. Therefore, the novel method could be a good candidate for ultra-sensitive and selective detection of Cronobacter species without complex manipulation.

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