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Establishment of a dual-signal sandwich sensor for detection of shigella sonnei based on truncated aptamers

文献类型： 外文期刊

作者： He, Yifei 1 ; Wei, Jinxiang 1 ; Ma, Pengfei 1 ; Khan, Imran Mahmood 1 ; Zhang, Lili 5 ; Sobia, Niazi 1 ; Xia, Yu 1 ; Wang, Zhouping 1 ;

作者机构： 1.Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China

2.Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Peoples R China

3.Jiangnan Univ, Int Joint Lab Food Safety, Wuxi 214122, Peoples R China

4.Jiangnan Univ, Natl Engn Res Ctr Funct Food, Wuxi 214122, Jiangsu, Peoples R China

5.Jiangsu Acad Agr Sci, Nanjing 210000, Jiangsu, Peoples R China

6.Jiangnan Univ, Collaborat Innovat Ctr Food Safety & Qual Control, Wuxi 214122, Peoples R China

7.Jiangnan Univ, 1800 Lihu Ave, Wuxi 214122, Jiangsu, Peoples R China

关键词： Shigella sonnei; Aptamer; Truncation; Colorimetric; SERS; Detection

期刊名称：FOOD CONTROL （ 影响因子：6.0； 五年影响因子：5.8 ）

ISSN： 0956-7135

年卷期： 2024 年 156 卷

页码：

收录情况： SCI

摘要： Shigella Sonnei (S. sonnei) is a common foodborne pathogen causing intestinal diseases. In this study, a sandwich structured colorimetric and surface-enhanced Raman scattering (SERS) dual methods adaptation sensor was prepared for the detection of S. sonnei. Firstly, two aptamers, SS4.1-2 (Kd = 43.5 +/- 2.78 nM) and SP1-2.2 (Kd = 23.3 +/- 3.01 nM) with good specificity and affinity were obtained by truncation strategy. Then, Fe3O4@SiO2@Ag nanoparticles were utilized to capture bacteria, while Cu-TCPP (Fe)/AuNPs nanosheets were used as signal probes due to their oxidase activity and SERS signals. The detection limits of the aptasensor were as low as 37 CFU/mL for the colorimetric signal and 7 CFU/mL for SERS signal. Furthermore, the aptasensor was successfully applied to detect S. sonnei in real samples with 94.95-112.92% and 98.40-103.95% recoveries for colorimetric and SERS methods, respectively. A more rapid and accurate method to detect S. sonnei contamination in food was improved.

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