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A DNA walker-driven SERS-fluorescent dual-mode aptasensor based on optimized aptamers to detect Shigella flexneri

文献类型： 外文期刊

作者： He, Yifei 1 ; Wei, Jinxiang 1 ; Zhang, Lili 4 ; Xia, Yu 1 ; Wang, Zhouping 1 ; Yang, Junsong 6 ;

作者机构： 1.Jiangnan Univ, State Key Lab Food Sci & Resources, Wuxi 214122, Peoples R China

2.Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Peoples R China

3.Jiangnan Univ, Int Joint Lab Food Safety, Wuxi 214122, Peoples R China

4.Jiangsu Acad Agr Sci, Gen Situat, Nanjing 210000, Jiangsu, Peoples R China

5.Jiangnan Univ, Collaborat Innovat Ctr Food Safety & Qual Control, Wuxi 214122, Peoples R China

6.BengBu Med Coll, Sch Publ Course, Teaching & Res Off Food Safety, Bengbu 233000, Peoples R China

7.Jiangnan Univ, 1800 Lihu Ave, Wuxi 214122, Jiangsu, Peoples R China

关键词： Foodborne pathogen; Aptasensor; SERS; Fluorescence; DNA walker

期刊名称：FOOD CONTROL （ 影响因子：6.0； 五年影响因子：5.8 ）

ISSN： 0956-7135

年卷期： 2024 年 160 卷

页码：

收录情况： SCI

摘要： In this study, a dual-mode SERS-fluorescent aptasensor based on a DNA walker was developed for Shigella flexneri (S. flexneri). Fe@SiO2@QDs were connected with H1 and the walking chain (hybrid strands of aptamer and cDNA) to fabricate fluorescent probes, and AgR6G@SiO2 were connected with H2 to fabricate SERS tags. Aptamer (Kd = 42.6 +/- 3.16 nM) selective binding of S.flexneri, left the walking chain. The Zn2+-specific DNAzyme in the cDNA was exposed thus driving the DNA walker. The sequence on H1 containing BHQ3 was cleaved from fluorescent probes to restore the fluorescence. The remaining H1 sequence could bind to the H2 to generate Raman signals. The detection limit of the fluorescence signal was 11 CFU/mL, and the Raman signal was 3 CFU/ mL. In addition, the aptasensor was successfully applied to the detection of actual samples with recovery ranges of 98.35-107.27% and 97.57-105.91% for the fluorescence and SERS methods, respectively.

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