文献类型： 外文期刊
作者： Gao, Jian-Jie 1 ; Wang, Bo 1 ; Li, Zhen-Jun 1 ; Xu, Jing 1 ; Fu, Xiao-Yan 1 ; Han, Hong-Juan 1 ; Wang, Li-Juan 1 ; Zhang, Wen-Hui 1 ; Deng, Yong-Dong 1 ; Wang, Yu 1 ; Gong, Ze-Hao 1 ; Tian, Yong-Sheng 1 ; Peng, Ri-He 1 ; Yao, Quan-Hong 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Biotechnol Res Inst, 2901 Beidi Rd, Shanghai 201106, Peoples R China; Shanghai Acad Agr Sci, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China
关键词： Thiocyanate; Phytoremediation; Thiocyanate hydrolase module; Metabolic engineering
期刊名称：SCIENCE OF THE TOTAL ENVIRONMENT （ 影响因子：10.753； 五年影响因子：10.237 ）
ISSN： 0048-9697
年卷期： 2022 年 820 卷
页码：
收录情况： SCI
摘要： Industrial thiocyanate (SCN-) waste streams from gold mining and coal coking have caused serious environmental pollution worldwide. Phytoremediation is an efficient technology in treating hazardous wastes from the environment. However, the phytoremediation efficiency of thiocyanate is very low due to the fact that plants lack thiocyanate degradation enzymes. In this study, the thiocyanate hydrolase module was assembled correctly in rice seedlings and showed thiocyanate hydrolase activity. Rice seedlings engineered to express thiocyanate degrading activity were able to completely remove thiocyanate from coking wastewater. Our findings suggest that transforming the thiocyanate hydrolase module into plants is an efficient strategy for rapid phytoremediation of thiocyanate in the environment. Moreover, the rice seedlings expressing apoplastic or cytoplasmic targeted thiocyanate hydrolase module were constructed to compare the phytoremediation efficiency of secretory/intracellular recombinant thiocyanate hydrolase. The most obvious finding from this study is that the apoplastic expression system is more efficient than the cytoplasm expression system in the phytoremediation of thiocyanate. At last, this research also shows that the secreted thiocyanate hydrolase from engineered rice plants does not influence rhizosphere bacterial community composition.
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