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Surface IgM lambda light chain is involved in the binding and infection of infectious bursal disease virus (IBDV) to DT40 cells

文献类型： 外文期刊

作者： Chi, Jiaqi 1 ; You, Leiming 2 ; Li, Peipei 3 ; Teng, Man 3 ; Zhang, Gaiping 3 ; Luo, Jun 3 ; Wang, Aiping 4 ;

作者机构： 1.Beijing Univ Chinese Med, Dongfang Hosp, Dept Oncol, Beijing 100078, Peoples R China

2.Beijing Univ Chinese Med, Sch Life Sci, Beijing 100029, Peoples R China

3.Henan Acad Agr Sci, Key Lab Anim Immunol, Henan Prov Key Lab Anim Immunol, Minist Agr, 116 Huayuan Rd, Zhengzhou 450002, Peoples R China

4.Zhengzhou Univ, Sch Life Sci, 100 Kexue Rd, Zhengzhou 450002, Henan, Peoples R China

关键词： DT40 cell; Infectious bursal disease virus; Lambda light chain; Small hairpin RNA; Surface immunoglobulin M

期刊名称：VIRUS GENES （ 影响因子：2.332； 五年影响因子：2.0 ）

ISSN： 0920-8569

年卷期： 2018 年 54 卷 2 期

页码：

收录情况： SCI

摘要： Infectious bursal disease virus (IBDV) is an important immunosuppressive virus in chickens. Surface immunoglobulin M (sIgM)-bearing B lymphocytes act as the major targets of IBDV in the bursa of Fabricius, and sIgM may function as one of the membrane binding sites responsible for IBDV infection. Recently, using the virus overlay protein binding assay, the chicken lambda light chain of sIgM was identified to specifically interact with IBDV in a virulence-independent manner in vitro. To further investigate sIgM lambda light chain-mediated IBDV binding and infection in pre-B cells, the cell line DT40, which is susceptible to both pathogenic and attenuated IBDV, was used. Based on the RNA interference strategy, the DT40 cell line whose lambda light chain of sIgM was stably knocked down, herein termed DT40LKD, was generated by the genomic integration of a specific small hairpin RNA and a green fluorescence protein co-expression construct. Flow cytometry analysis indicated that the binding of IBDV to DT40LKD cells was significantly reduced due to the loss of sIgM lambda light chain. In particular, reduced viral replication was observed in IBDV-incubated DT40LKD cells, and no viral release into cell culture medium was detected by the IBDV rapid diagnostic strips. In addition, the rescue of sIgM lambda light chain expression restored viral binding and replication in DT40LKD cells. These results show that sIgM lambda light chain appears to be beneficial for IBDV attachment and infection, suggesting that sIgM acts as a binding site involved in IBDV infection.

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