A fluorescein-based "turn-on" fluorescence probe for hypochlorous acid detection and its application in cell imaging
文献类型： 外文期刊
作者： Li, Jie 1 ; Yang, Xiaopeng 1 ; Zhang, Di 2 ; Liu, Yongxiang 1 ; Tang, Jun 1 ; Li, Yan; Zhao, Yufen 1 ; Ye, Yong 1 ;
作者机构： 1.Zhengzhou Univ, Coll Chem & Mol Engn, Phosphorus Chem Engn Res Ctr Henan Prov, Zhengzhou 450001, Henan, Peoples R China
2.Henan Acad Agr Sci, Inst Agr Qual Stand & Testing Technol, Zhengzhou 450002, Henan, Peoples R China
3.Xiamen Univ, Coll Chem & Chem Engn, Key Lab Chem Biol Fujian Prov, Xiamen 361005, Peoples R China
4.Xiamen Univ, Coll Chem & Chem Engn, Key Lab Chem Biol Fujian Prov, Xiamen 361005, Peoples R China;
关键词： Fluorescence probe; Fluorescein; C=N isomerization; HC1O; Cell imaging
期刊名称：SENSORS AND ACTUATORS B-CHEMICAL （ 影响因子：7.46； 五年影响因子：6.743 ）
ISSN： 0925-4005
年卷期： 2018 年 265 卷
页码：
收录情况： SCI
摘要： A simple fluorescent probe F-BH was designed and synthesized, introducing C=N group which can be a recognition site for hypochlorous acid into the fluorescent probe molecule by using 2-benzothiazole hydrazone and formylated fluorescein. The fluorescence intensity of the probe F-BH solution was very weak, while the fluorescence of the solution was obviously enhanced and the color of the solution changed after the addition of C10(-) ion. The selection and competition experiments proved that the probe has great selectivity for recognition of hypochlorous acid over other ROSs/RNSs and anions. Fluorescence titration experiments show that the probe has a low detection limit (17.5 nM). The probe has been successfully applied to real-time detection of hypochlorous acid in Hela cells. (C) 2018 Elsevier B.V. All rights reserved.
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