Novel monoclonal antibody-based immunochromatographic strip for detecting citrinin in fruit from Zhejiang province, China
文献类型： 外文期刊
作者： Cheng, Haiwei 1 ; Yang, Yi 1 ; Chen, Yifei 1 ; Chen, Xueqiu 1 ; Cai, Zizheng 5 ; Du, Aifang 1 ;
作者机构： 1.Zhejiang Univ, Coll Anim Sci, Inst Prevent Vet Med, Hangzhou, Zhejiang, Peoples R China
2.Zhejiang Univ, Coll Anim Sci, Zhejiang Prov Key Lab Prevent Vet Med, Hangzhou, Zhejiang, Peoples R China
3.Jiangsu Acad Agr Sci, Natl Res Ctr Engn & Technol Vet Biol, Key Lab Vet Biol Engn & Technol, Minist Agr, Nanjing, Jiangsu, Peoples R China
4.Jiangsu Coinnovat Ctr Prevent & Control Import An, Yangzhou, Jiangsu, Peoples R China
5.Nanjing Agr Univ, Nanjing, Jiangsu, Peoples R China
期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）
ISSN： 1932-6203
年卷期： 2018 年 13 卷 5 期
页码：
收录情况： SCI
摘要： Citrinin (CIT) is a hepato-nephrotoxic fungal metabolite produced by the genera Penicillium, Aspergillus and Monascu. There is an increasing demand for rapid and economical methods for detection CIT residues in fruit. In this study, we developed an immunochromatographic strip (ICS) for detection of citrinin (CIT) residues in fruit for the first time. Anti-CIT monoclonal antibody (McAb) 2B9 was prepared, with a binding affinity of 9.39 x 10(8) L/moL. Conjugates CIT-BSA and McAb 2B9 were used to develop the ICS which could be completed in 5 min, with the detection limit of 50 ng/mL and no cross reactivity with other mycotoxins. Analysis of CIT in 64 fruit samples revealed that data obtained from the ICS test were in good agreement with indirect competitive enzyme-linked immunosorbent assays (ic-ELISAs) and high performance liquid chromatography (HPLC). This result demonstrated that the ICS test could be used as a rapid, reliable, cost-effective and user-friendly qualitative tool for detection of CIT residues on-site.
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