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Development of an immunochromatographic lateral flow strip for the simultaneous detection of aminoglycoside residues in milk

文献类型： 外文期刊

作者： Sun, Yaning 1 ; Yang, Jifei 1 ; Yang, Suzhen 1 ; Sang, Qingbo 4 ; Teng, Man 1 ; Li, Qingmei 1 ; Deng, Ruiguang 1 ; Feng, 1 ;

作者机构： 1.Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Minist Agr, Key Lab Anim Immunol, Zhengzhou 450002, Henan, Peoples R China

2.Henan Acad Agr Sci, Inst Agr Econ & Informat, Zhengzhou 450002, Henan, Peoples R China

3.Henan Agr Univ, Coll Anim Sci & Vet Med, 63 Nongye Rd, Zhengzhou 450002, Henan, Peoples R China

4.Zhengzhou Ruilong Food Sci & Technol Ltd Co, Zhengzhou 450064, Henan, Peoples R China

期刊名称：RSC ADVANCES （ 影响因子：3.361； 五年影响因子：3.39 ）

ISSN： 2046-2069

年卷期： 2018 年 8 卷 17 期

页码：

收录情况： SCI

摘要： A colloidal gold-based immunochromatographic strip with a competitive format has been developed for the rapid, simultaneous, semi-quantitative and quantitative detection of several aminoglycoside residues in milk, including gentamicin sulfate (GM), neomycin sulfate (NEO) and kanamycin sulfate (KN). Three monoclonal antibodies against the three corresponding aminoglycosides were conjugated to colloidal gold particles and applied to the conjugate pads of the strip. The competitors [GM-bovine serum albumin (GM-BSA), NEO-BSA and KN-BSA conjugates] of GM, NEO and KN were immobilized onto a nitrocellulose (NC) membrane at three detection zones, T1, T2, and T3, respectively. The minimal cutoff values of the strip were 10 ng mL(-1) for GM, and 100 ng mL(-1) for NEO and KN, which are lower than the maximum residue levels (MRLs) established for aminoglycosides. The IC50 values of the strip were 0.737 ng mL(-1), 8.971 ng mL(-1) and 11.110 ng mL(-1) for GM, NEO and KN respectively. In conclusion, the immunochromatographic lateral flow strip could be used for rapid, simultaneous, semi-quantitative and quantitative detection of GM, NEO and KN residues in milk.

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