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A lateral flow immunochromatographic strip test for rapid detection of hexoestrol in fish samples

文献类型： 外文期刊

作者： Yang, Xingdong 1 ; Sun, Zhongke 1 ; Tian, Fengshou 1 ; Jia, Guochao 3 ; Yang, Jifei 2 ; Hu, Xiaofei 2 ;

作者机构： 1.Zhoukou Normal Univ, Inst Food & Drug Inspect, Zhoukou 466001, Peoples R China

2.Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Minist Agr, Key Lab Anim Immunol, Zhengzhou 450002, Henan, Peoples R China

3.Univ Shanghai Sci & Technol, Sch Med Instrument & Food Engn, Shanghai 200093, Peoples R China

关键词： hexoestrol; immunoassay; colloidal gold; lateral flow immunochromatographic strip test (LFIST); rapid test

期刊名称：ROYAL SOCIETY OPEN SCIENCE （ 影响因子：2.963； 五年影响因子：3.44 ）

ISSN： 2054-5703

年卷期： 2018 年 5 卷 8 期

页码：

收录情况： SCI

摘要： A lateral flow immunochromatographic strip test was developed for rapid and sensitive on-site detection of hexoestrol (HES) residues in fish samples with colloidal gold labelling of the anti-HES monoclonal antibody. The strip is composed of a sample pad, a conjugate reagent pad, an absorbent pad and a test membrane containing a control line and a test line. The sensitivity (half inhibitory concentration, IC50) of the strip in the detection of fish extract samples was confirmed to be 1.86 mg kg(-1), and the limit of detection value was 0.62 mg kg(-1). For intra-assay and inter-assay reproducibility, recoveries of HES-spiked samples ranged from 86.3% to 92.3% and 85.8% to 93.4%, coefficients of variation were 2.91-4.64% and 4.24-5.17%, respectively. High-performance liquid chromatography was employed to confirm the performance of the strip. The strip test takes less than 10 min, and thus provides a repaid method for on-site detection of HES residues.

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