Efficient transformation of Pleurotus eryngii with a safe selective marker mutated from the Pesdi1 gene
文献类型： 外文期刊
作者： Shang, Junjun 1 ; Li, Yan 1 ; Yang, Ruiheng 1 ; Wang, Ying 1 ; Mao, Wenjun 1 ; Tang, Lihua 1 ; Wu, Yingying 1 ; Nakazawa, 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Shanghai Key Lab Agr Genet & Breeding, Inst Edible Fungi, 1000 Jingqi Rd, Shanghai 201403, Peoples R China
2.Shanghai Acad Agr Sci, Shanghai Key Lab Agr Genet & Breeding, Inst Edible Fungi, 1000 Jingqi Rd, Shanghai 2
关键词： Pleurotus eryngii; Transformation; Selective marker; Carboxin
期刊名称：JOURNAL OF MICROBIOLOGICAL METHODS （ 影响因子：2.363； 五年影响因子：2.24 ）
ISSN： 0167-7012
年卷期： 2018 年 152 卷
页码：
收录情况： SCI
摘要： We introduced a site-directed mutation in the sdi1 gene and used it as a selective marker for the polyethylene glycol-mediated transformation of Pleurotus eryngii monokaryon protoplasts. The transformants displayed obvious and stable resistance to the fungicide carboxin indicating that the mutant Pesdil gene is an efficient selective marker.
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