Efficient transformation of Pleurotus eryngii with a safe selective marker mutated from the Pesdi1 gene
文献类型： 外文期刊
作者： Shang, Junjun 1 ; Li, Yan 1 ; Yang, Ruiheng 1 ; Wang, Ying 1 ; Mao, Wenjun 1 ; Tang, Lihua 1 ; Wu, Yingying 1 ; Nakazawa, 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Shanghai Key Lab Agr Genet & Breeding, Inst Edible Fungi, 1000 Jingqi Rd, Shanghai 201403, Peoples R China
2.Shanghai Acad Agr Sci, Shanghai Key Lab Agr Genet & Breeding, Inst Edible Fungi, 1000 Jingqi Rd, Shanghai 2
关键词： Pleurotus eryngii; Transformation; Selective marker; Carboxin
期刊名称：JOURNAL OF MICROBIOLOGICAL METHODS （ 影响因子：2.363； 五年影响因子：2.24 ）
ISSN： 0167-7012
年卷期： 2018 年 152 卷
页码：
收录情况： SCI
摘要： We introduced a site-directed mutation in the sdi1 gene and used it as a selective marker for the polyethylene glycol-mediated transformation of Pleurotus eryngii monokaryon protoplasts. The transformants displayed obvious and stable resistance to the fungicide carboxin indicating that the mutant Pesdil gene is an efficient selective marker.
- 相关文献
作者其他论文 更多>>
-
Synergizing postharvest physiology and nanopackaging for edible mushroom preservation
作者：Gong, Ming;Zhang, Tongyan;Wu, Yingying;Shang, Junjun;Gong, Ming;Su, Erzheng;Cao, Yu;Gong, Ming;Zhang, Tongyan;Zhang, Jianguo
关键词：Edible fungi; Postharvest preservation; Physiological changes; Nanopackaging; Food nanotechnology
-
Effectiveness of Different Organic Solvent Additions to Water Samples for Reducing the Adsorption Effects of Organic Pesticides Using Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry
作者：Liu, Yucan;Xu, Xinyi;Wang, Ying;Zhang, Yan;Lu, Jianbo;Liu, Chengbin;Duan, Jinming;Sun, Hongwei
关键词：adsorption effect; UHPLC-ESI-MS/MS; organic pesticides; direct injection technique; detection signal intensity; organic solvent; addition ratio
-
OsMAPKKK5 affects brassinosteroid signal transduction via phosphorylating OsBSK1-1 and regulates rice plant architecture and yield
作者：Yan, Peiwen;Wang, Ying;Cui, Jinhao;Liu, Mingyu;Zhu, Yu;Ma, Fuying;Liu, Yahui;Lan, Dengyong;Dong, Shiqing;Zhang, Xinwei;He, Shicong;Hu, Jian;Yuan, Xinyu;Li, Yizhen;Yang, Jinshui;Luo, Xiaojin;Yan, Peiwen;Wang, Ying;Cui, Jinhao;Liu, Mingyu;Zhu, Yu;Ma, Fuying;Liu, Yahui;Lan, Dengyong;Dong, Shiqing;Zhang, Xinwei;He, Shicong;Hu, Jian;Yuan, Xinyu;Li, Yizhen;Yang, Jinshui;Luo, Xiaojin;Wang, Ying;Wang, Ying;Hu, Zejun;Niu, Fuan;Cao, Liming;Liu, Yang;Luo, Xiaojin
关键词：brassinosteroids; mitogen-activated protein kinase kinase kinase; crosstalk; plant architecture; rice yield
-
Multiple Analyses Reveal Evidence for Three New Species of Collybia (Clitocybaceae, Basidiomycete) from China
作者：Qi, Yue;Yang, Liu;Guo, Yaobin;Wan, Fashuang;Yu, Xiaodan;Xu, Aiguo;Guo, Hongbo;Yang, Ruiheng;Pei, Ying
关键词：mushrooms; new taxa; morphological features; phylogenetic relationships
-
Editing a mushroom with high-digestibility using a novel endo-N-acetyl-β-D-glucosaminidase
作者：Wang, Ying;Chen, Wenjing;Chen, Hongyu;Gong, Ming;Shao, Youran;Bao, Dapeng;Zou, Gen;Chen, Wenjing;Wang, Li
关键词：Mycoprotein; CRISPR/Cas9; Molecular design breeding; Endo; N -acetyl-beta-D-glucosaminidase; Cordyceps militaris
-
Characterization and Functional Analysis of a Novel Fungal Immunomodulatory Protein Gene from Ganoderma leucocontextum in B16-F10 Mouse Melanoma Cells
作者：Yang, Jiayi;Jin, Mengyuan;Zhang, Lida;Zhou, Xuanwei;Wu, Yingying
关键词：
Ganoderma leucocontextum ; fungal immunomodulatory proteins; protein expression; B16-F10 melanoma cell -
The Exploration of Cordyceps militaris Extract as a Postharvest Preservative for Flammulina filiformis
作者：Chen, Wenjing;Chen, Wenjing;Li, Yan;Shao, Youran;Chen, Hongyu;Gong, Ming;Wang, Ying;Zou, Gen;Wang, Li;Li, Nanyi
关键词：
Flammulina filiformis ;Cordyceps militaris ; bacteriostasis; antioxidation; preservation
