A Lateral Flow lmmunochromato-graphic Strip Test for Rapid Detection of Oseltamivir Phosphate in Egg and Chicken Meat
文献类型： 外文期刊
作者： Yang, Xingdong 1 ; Yang, Jifei 2 ; Wang, Yinbiao 3 ; Li, Lili 1 ; Sun, Zhongke 1 ; Yue, Zonghao 1 ; Tian, Fengshou 1 ; He 1 ;
作者机构： 1.Zhoukou Normal Univ, Inst Food & Drug Inspect, Zhoukou 466001, Peoples R China
2.Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Minist Agr, Key Lab Anim Immunol, Zhengzhou 450002, Henan, Peoples R China
3.Xinxiang Med Univ, Sch Publ Hlth, Xinxiang 453003, Peoples R China
期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）
ISSN： 2045-2322
年卷期： 2018 年 8 卷
页码：
收录情况： SCI
摘要： A lateral flow immunochromatographic strip test (LFIST) based on a competitive format was developed for rapid and sensitive on-site detection of oseltamivir phosphate (OP) residues in poultry product. The sensitivity (half inhibitory concentration, IC50) of the LFIST in the detection of egg and chicken meat samples was confirmed to be 2.56 and 2.63 mu g/kg, and the limit detection (LOD) value were 0.43 and 0.42 mu g/kg, respectively. For intra-assay and inter-assay reproducibility, recoveries of OP spiked samples ranged between 82.8% and 91.2% with coefficients of variations (CV) less than 5.67% (intraassay) and 6.52% (inter-assay). The performance of LFIST was comparable to high-performance liquid chromatography (HPLC) in a parallel testing of egg samples and chicken samples. LFIST takes less than 5 minutes, eliminates the dependency on professional personnel, and thus can be used as a surveillance tool for on-site detection of OP residues.
- 相关文献
作者其他论文 更多>>
-
Development of an Enzyme-Linked Immunosorbent Assay Based on a Monoclonal Antibody for the Rapid Detection of Citrinin in Wine
作者：Yang, Xingdong;Qu, Yang;Wang, Chenchen;Wu, Lihua;Yang, Xingdong;Hu, Xiaofei;Yang, Xingdong;Hu, Xiaofei
关键词：citrinin; monoclonal antibody; ic-ELISA; wine
-
An indirect competitive ELISA for determination of guanidine acetic acid in animal feed
作者：Yang, Xingdong;Li, Lili;Qu, Yang;Wu, Lihua;Wang, Chenchen;He, Beibei;Zang, Zhenchun;Yang, Xingdong;Hu, Xiaofei
关键词：Guanidino acetic acid; Immunogen; Monoclonal antibody; ELISA; Animal feed
-
Development of an ultrasensitive indirect competitive chemiluminescent enzyme-linked immunoassay for the rapid detection of bacitracin in animal tissues and milk
作者：Yang, Xingdong;Beibei, He;Qu, Yang;Wu, Lihua;Wang, Chenchen;Cao, Yutong;Zhu, Yinuo;Zang, Zhenchun;Li, Lili;Yang, Xingdong;Hu, Xiaofei;Li, Lili
关键词：Bacitracin; Monoclonal antibody; Enzyme-linked chemiluminescent; Immunoassay; Milk; Animal tissue
-
Identification of the linear Fc-binding epitope on the bovine IgG1 Fc receptor of (boFcγRI) using synthetic peptides
作者：Li, Qingmei;Wang, Ruining;Yang, Jifei;Guo, Junqing;Zhang, Gaiping;Li, Ge;Wang, Xun;Zhang, Gaiping;Wang, Ruining;Zhang, Gaiping
关键词：Fc-binding epitope; BoFc gamma RI; Bovine IgG1; Synthetic peptides; Binding inhibition
-
An immunochromatographic strip sensor for marbofloxacin residues
作者：Yang, Xingdong;Zhang, Qianqian;Yang, Xingdong;Li, Qingmei;Yang, Jifei;Hu, Xiaofei;Kwee, Sharon
关键词：
-
Application of Surface-Enhanced Raman Spectroscopy Combined with Immunoassay for the Detection of Adrenoceptor Agonists
作者：Wang, Yao;Jing, Yubing;Cao, Jinbo;Sun, Yingying;Guo, Kaitong;Chen, Xiujin;Li, Zhaozhou;Cao, Jinbo;Guo, Kaitong;Hu, Xiaofei;Shi, Qiaoqiao
关键词：adrenoceptor agonists; SERS; immunoassays; substrate materials; Raman reporter molecules
-
Establishment of a Chemiluminescent ELISA Method for Florfenicol in Eggs and Chicken Meat
作者：Ge, Mengjun;Cai, Wenjin;Wang, Yao;Ge, Mengjun;Xing, Yunrui;Sun, Yaning;Cai, Wenjin;Hu, Xiaofei
关键词：Florfenicol; Hazard; Ci-ELISA; Egg; Chicken meat
