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A Lateral Flow lmmunochromato-graphic Strip Test for Rapid Detection of Oseltamivir Phosphate in Egg and Chicken Meat

文献类型： 外文期刊

作者： Yang, Xingdong 1 ; Yang, Jifei 2 ; Wang, Yinbiao 3 ; Li, Lili 1 ; Sun, Zhongke 1 ; Yue, Zonghao 1 ; Tian, Fengshou 1 ; He 1 ;

作者机构： 1.Zhoukou Normal Univ, Inst Food & Drug Inspect, Zhoukou 466001, Peoples R China

2.Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Minist Agr, Key Lab Anim Immunol, Zhengzhou 450002, Henan, Peoples R China

3.Xinxiang Med Univ, Sch Publ Hlth, Xinxiang 453003, Peoples R China

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2018 年 8 卷

页码：

收录情况： SCI

摘要： A lateral flow immunochromatographic strip test (LFIST) based on a competitive format was developed for rapid and sensitive on-site detection of oseltamivir phosphate (OP) residues in poultry product. The sensitivity (half inhibitory concentration, IC50) of the LFIST in the detection of egg and chicken meat samples was confirmed to be 2.56 and 2.63 mu g/kg, and the limit detection (LOD) value were 0.43 and 0.42 mu g/kg, respectively. For intra-assay and inter-assay reproducibility, recoveries of OP spiked samples ranged between 82.8% and 91.2% with coefficients of variations (CV) less than 5.67% (intraassay) and 6.52% (inter-assay). The performance of LFIST was comparable to high-performance liquid chromatography (HPLC) in a parallel testing of egg samples and chicken samples. LFIST takes less than 5 minutes, eliminates the dependency on professional personnel, and thus can be used as a surveillance tool for on-site detection of OP residues.

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