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Food-grade gene transformation system constructed in Lactobacillus plantarum using a GlmS-encoding selection marker

文献类型： 外文期刊

作者： Chen, Ying 1 ; Qi, Min 1 ; Xu, Mengqiu 1 ; Huan, Hailin 2 ; Shao, Weilan 3 ; Yang, Yao 1 ;

作者机构： 1.Nanjing Normal Univ, Dept Food Sci & Technol, Jinling Coll, Nanjing 210097, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Anim Sci, Nanjing 210014, Jiangsu, Peoples R China

3.Jiangsu Univ, Biofuels Inst, Sch Environm & Safety Engn, Zhenjiang 212013, Jiangsu, Peoples R China

关键词： food-grade vector; glmS; ldhL promoter; lactic acid bacteria

期刊名称：FEMS MICROBIOLOGY LETTERS （ 影响因子：2.742； 五年影响因子：2.856 ）

ISSN： 0378-1097

年卷期： 2018 年 365 卷 21 期

页码：

收录情况： SCI

摘要： Food-grade gene expression systems in lactic acid bacteria enable production of functional proteins or product testing without antibiotic requirement. Here, we expanded the available selection markers by developing a novel food-grade genetic transformation system for Lactobacillus plantarum WCFS1 using the glucosamine-6-phosphate synthase gene (glmS1). A glmS-vector pSIPH497 was constructed by replacing the erythromycin resistance gene (erm) with L. plantarum glmS1 under control of the P-ldhL promoter from WCFS1. The selection efficiency and stability of the glmS-vector were shown to be comparable to those of the erm-based plasmid. Moreover, using mCherry expression as a reporter gene, we showed the feasibility of the system for producing foreign proteins. This food-grade host/vector system will provide an effective and safe technique for the application of lactic acid bacteria in the food and medical industries. Furthermore, this study provides a useful strategy for developing food-grade selection markers in other host/vector systems.

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