Regulation of antimicrobial peptide genes via insulin-like signaling pathway in the silkworm Bombyx mori
文献类型： 外文期刊
作者： Zhang, Jie 1 ; Yang, Weike 1 ; Xu, Junfeng 1 ; Yang, Wanying 1 ; Li, Qingrong 2 ; Zhong, Yangjin 1 ; Cao, Yang 1 ; Yu, Xi 1 ;
作者机构： 1.South China Agr Univ, Coll Anim Sci, Guangdong Prov Sericulture & Mulberry Engn Res Ct, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Guangdong, Peoples R China
2.Guangdong Acad Agr Sci, Sericultural & Agri Food Res Inst, Guangzhou 510610, Guangdong, Peoples R China
3.Univ Missouri, Sch Biol Sci, Div Mol Biol & Biochem, 5007 Rockhill Rd, Kansas City, MO 64110 USA
关键词： Akt; Antimicrobial peptide; FoxO; Insulin-like signaling; Starvation
期刊名称：INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY （ 影响因子：4.714； 五年影响因子：4.953 ）
ISSN： 0965-1748
年卷期： 2018 年 103 卷
页码：
收录情况： SCI
摘要： Antimicrobial peptides (AMPs) are important effector molecules of insect humoral immunity, and expression of AMPs is mainly regulated by the Toll and immune deficiency (IMD) pathways. FoxO, a key downstream regulator of the insulin-like signaling (ILS) pathway, has been recently reported to be involved in the regulation of AMPs in Drosophila melanogaster. In the present study, we investigated AMP gene expression and the regulation pathway controlled by the starvation in the silkworm Bombyx mori. We discovered that antibacterial activity in the hemolymph of B. mori larvae was increased by starvation, and expression of AMP genes (BmCecB6, BmAtta1, BmLeb3 and BmDefB) as well as the ILS target genes (FoxO, InR and Brummer) were strongly activated in the fat body by starvation. Moreover, phosphorylation of Akt kinase was reduced in the Bm-12 cells after starvation, suggesting that the ILS pathway was inhibited by starvation. We then showed that more FoxO protein was present in the cytoplasm than in the nucleus of Bm-12 cells under normal conditions, but more FoxO was detected in the nucleus after cells were starved for 8 h, indicating that FoxO was activated by starvation. In summary, our results indicated that starvation can activate AMP gene expression in B. mori via the ILS/FoxO signaling pathway.
- 相关文献
作者其他论文 更多>>
-
Dietary supplementation of mulberry leaf oligosaccharides improves the growth, glucose and lipid metabolism, immunity, and virus resistance in largemouth bass (Micropterus salmoides)
作者：Zhou, Donglai;Zhong, Wenhao;Fu, Bing;Li, Erna;Li, Qingrong;Yang, Qiong;Zou, Yuxiao;Liao, Sentai;Xing, Dongxu;Zhou, Donglai;Li, Erna;Wang, Fubao;Zhong, Wenhao;Hao, Le;Liu, Zhenxing
关键词：largemouth bass; growth; serum biochemistry; liver metabolism; inflammation
-
Mulberry leaf extract reduces damage by regulating the oxidative response, immune response and intestinal flora of largemouth bass (Micropterus salmoides) under chronic heat stress
作者：Fu, Bing;Zhou, Donglai;Qiu, Xiaotong;Zheng, Jieqiu;Yang, Qiong;Xiao, Yang;Liao, Sentai;Li, Qingrong;Xing, Dongxu;Qiu, Xiaotong;Zheng, Jieqiu
关键词：mulberry leaf extract; largemouth bass; heat stress; intestinal flora; liver injury; immune ability
-
Hsp70-mediated manipulation of antimicrobial peptide-related genes promotes BmNPV proliferation in Bombyx mori at relatively high temperatures
作者：Zhang, Jie;Hu, Yingying;Feng, Jiawei;He, Na;Li, Xiaofeng;Liyi, Qingge;Yi, Huiyu;Yang, Wanying;Zhang, Jie;Yang, Qiong
关键词：Bombyx mori; BmNPV; Innate immunity; Host-pathogen interaction; Heat shock protein (Hsp); Antimicrobial peptide (AMP)
-
Anti-Hyperuricemic and Nephroprotective Effects of Hydrolysate Derived from Silkworm Pupae (Bombyx mori): In Vitro and In Vivo Study
作者：Fan, Yuting;Yang, Zhencong;Lin, Xiao;Xu, Zhoujin;Wu, Xuli;Mu, Lixia;Li, Qingrong
关键词：silkworm pupae; peptides; anti-hyperuricemic; anti-inflammatory; xanthine oxidase
-
Rheological Behavior, Textural Properties, and Antioxidant Activity of Porphyra yezoensis Polysaccharide
作者：Ji, Chenyang;Long, Xiaoshan;Qi, Bo;Hu, Xiao;Ji, Chenyang;Hu, Xiao;Ji, Chenyang;Wang, Jingjie;Cao, Yang;Wang, Jingjie;Cao, Yang;Qi, Bo;Hu, Xiao
关键词：
Porphyra yezoensis polysaccharide; rheological properties; gelation mechanism; calcium ions; antioxidant activities -
Effects of Pile-Fermentation Duration on the Taste Quality of Single-Cultivar Large-Leaf Dark Tea: Insights from Metabolomics and Microbiomics
作者：Yang, Wanying;Ni, He;Chen, Ruohong;Sun, Lingli;Li, Qiuhua;Lai, Xingfei;Zhang, Zhenbiao;Lai, Zhaoxiang;Hao, Mengjiao;Sun, Shili;Li, Qian;Lin, Sen
关键词：dark tea; quality; taste; pile-fermentation; metabolomics; microbiomics
-
Transcriptomics and Metabolomics Combined to Analyze the Response Mechanism of Silkworm Eggs to High-Temperature Stress
作者：Xiao, Yang;Li, Qingrong;Sun, Zhenbo;Fu, Bing;Yang, Qiong;Zhang, Weilong;Xing, Dongxu;Jiang, Mangui;Huang, Xuhua
关键词：transcriptomics; metabonomics; silkworm egg; instant acid-impregnated eggs; high temperature stress
