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Heterologous Expression and Characterization of a Laccase from Laccaria bicolor in Pichia pastoris and Arabidopsis thaliana

文献类型： 外文期刊

作者： Wang, Bo 1 ; Yan, Ying 2 ; Xu, Jing 1 ; Fu, Xiaoyan 1 ; Han, Hongjuan 1 ; Gao, Jianjie 1 ; Li, Zhenjun 1 ; Wang, Lijuan 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Agrobiotechnol Res Inst, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China

2.Shanghai Acad Agr Sci, Crop Breeding & Cultivat Res Inst, Shanghai 201403, Peoples R China

关键词： Laccase; Laccaria bicolor; heterologous expression; Pichia pastoris; Arabidopsis thaliana

期刊名称：JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：2.351； 五年影响因子：2.65 ）

ISSN： 1017-7825

年卷期： 2018 年 28 卷 12 期

页码：

收录情况： SCI

摘要： Laccases can oxidize a variety of phenolic and non-phenolic substrates including synthetic dyes. In this research, a laccase gene Lcc9 from Laccaria bicolor was chemically synthesized and optimized to heterogeneous expression in Pichia pastoris and Arabidopsis thaliana. The properties of recombinant laccase expressed by P. pastoris were investigated. The laccase activity was optimal at 3.6 pH and 40 degrees C. It exhibited K-m and V-max values of 0.565 mmol l(-1) and 1.51 mu mol l(-1) min(-1) for ABTS respectively. As compared with untransformed control plants, the laccase activity in crude extracts of transgenic lines exhibited a 5.4 to 12.4-fold increase. Both laccases expressed in transgenic P. pastoris or A. thaliana could decolorize crystal violet. These results indicated that L. bicolor laccase gene may be transgenically exploited in fungi or plants for dye decolorization.

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