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Cloning, tissue distribution and methylation analyses of Lhx9 in Chinese tongue sole (Cynoglossus semilaevis)

文献类型： 外文期刊

作者： Zhu, Ying 1 ; Xu, Wenteng 1 ; Wang, Shuangyan 1 ; Gan, Tian 1 ; Shao, Changwei 1 ; Li, Ming 1 ; Wang, Na 1 ; Chen, Songli 1 ;

作者机构： 1.Minist Agr, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, CAFS, Qingdao 266071, Peoples R China; Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China

关键词： Lhx9; Gonad; Expression; ISH; Methylation

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN： 0378-1119

年卷期： 2019 年 691 卷

页码：

收录情况： SCI

摘要： Lhx9 is a LIM-homeodomain protein related to gonad development and sex reversal. In this study, we cloned and characterized CS-Lhx9 in the gonads of the Chinese tongue sole (Cynoglossus semilaevis). The full-length cDNA of CS-Lhx9 was 3123 bp, including an ORF of 1149 bp encoding 383 amino acids which contains two LIM domains and a homeobox domain. CS-Lhx9 transcripts were primarily observed in the testis of male and neomale at 1 yah, but nearly undetectable in the ovary. During the development of gonad, CS-Lhx9 exhibited an increasing trend and appeared to reach its peak value of expression in testis at 1 yah. In situ hybridization was performed in male and neomale gonads at 210 dah and 1 yah. The results showed strong CS-Lhx9 signals in the spermatids and spermatozoa (germ cells). Methylation level of CS-Lhx9 promoter was higher in female and lower in male and neomale gonads, showing a negative correlation with CS-Lhx9 expression.

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