文献类型： 外文期刊
作者： Tao, Yajun 1 ; Wang, Jun 2 ; Miao, Jun 1 ; Chen, Jie 1 ; Wu, Shujun 3 ; Zhu, Jinyan 1 ; Zhang, Dongping 1 ; Gu, Houwen 1 ; C 1 ;
作者机构： 1.Yangzhou Univ, Jiangsu Key Lab Crop Genet & Physiol, Coinnovat Ctr Modern Prod Technol Grain Crops, Key Lab Plant Funct Genom,Minist Educ,Jiangsu Key, Yangzhou 225009, Jiangsu, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Food Crops, Nanjing 210014, Peoples R China
3.Shanghai Acad Agr Sci, Shanghai 201403, Peoples R China
期刊名称：PLANT PHYSIOLOGY （ 影响因子：8.34； 五年影响因子：8.972 ）
ISSN： 0032-0889
年卷期： 2018 年 178 卷 4 期
页码：
收录情况： SCI
摘要： Polyamines, including putrescine, spermidine, and spermine, play essential roles in a wide variety of prokaryotic and eukaryotic organisms. Rice (Oryza sativa) contains four putative spermidine/spermine synthase (SPMS)-encoding genes (OsSPMS1, OsSPMS2, OsSPMS3, and OsACAULIS5), but none have been functionally characterized. In this study, we used a reverse genetic strategy to investigate the biological function of OsSPMS1. We generated several homozygous RNA interference (RNAi) and overexpression (OE) lines of OsSPMS1. Phenotypic analysis indicated that OsSPMS1 negatively regulates seed germination, grain size, and grain yield per plant. The ratio of spermine to spermidine was significantly lower in the RNAi lines and considerably higher in the OE lines than in the wild type, suggesting that OsSPMS1 may function as a SPMS. S-Adenosyl-L-methionine is a common precursor of polyamines and ethylene biosynthesis. The 1-aminocyclopropane-1-carboxylic acid (ACC) and ethylene contents in seeds increased significantly in RNAi lines and decreased in OE lines, respectively, compared with the wild type. Additionally, the reduced germination rates and growth defects of OE lines could be rescued with ACC treatment. These data suggest that OsSPMS1 affects ethylene synthesis and may regulate seed germination and plant growth by affecting the ACC and ethylene pathways. Most importantly, an OsSPMS1 knockout mutant showed an increase in grain yield per plant in a high-yield variety, Suken118, suggesting that OsSPMS1 is an important target for yield enhancement in rice.
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