您好，欢迎访问湖北省农业科学院 机构知识库！
湖北省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Characterization of multiple type-VI secretion system (T6SS) VgrG proteins in the pathogenicity and antibacterial activity of porcine extra-intestinal pathogenic Escherichia coli

文献类型： 外文期刊

作者： Zong, Bingbing 1 ; Zhang, Yanyan 1 ; Wang, Xiangru 1 ; Liu, Manli 5 ; Zhang, Tongchao 1 ; Zhu, Yongwei 1 ; Zheng, Yuch 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Wuhan, Hubei, Peoples R China

2.Cooperat Innovat Ctr Sustainable Pig Prod, Key Lab Prevent Vet Med Hubei Prov, Wuhan, Hubei, Peoples R China

3.Minist Agr Peoples Republ China, Key Lab Dev Vet Diagnost Prod, Wuhan, Hubei, Peoples R China

4.Minist Sci & Technol Peoples Republ China, Int Res Ctr Anim Dis, Wuhan, Hubei, Peoples R China

5.Hubei Acad Agr Sci, Hubei Biopesticide Engn Res Ctr, Wuhan, Hubei, Peoples R China

关键词： Porcine ExPEC PCN033; antibacterial activity; pathogenicity; T6SS; VgrG; gene deletion

期刊名称：VIRULENCE （ 影响因子：5.882； 五年影响因子：6.489 ）

ISSN： 2150-5594

年卷期： 2019 年 10 卷 1 期

页码：

收录情况： SCI

摘要： Porcine extra-intestinal pathogenic Escherichia coli (ExPEC) causes great economic losses to the pig industry and poses a serious threat to public health worldwide. Some secreted virulence factors have been reported to be involved in the pathogenicity of the infection caused by ExPEC. Type-VI secretion system (T6SS) is discovered in many Gram-negative bacteria and contributes to the virulence of pathogenic bacteria. Valine-glycine repeat protein G (VgrG) has been reported as an important component of the functional T6SS. In our previous studies, a functional T6SS was identified in porcine ExPEC strain PCN033. Further analysis of the PCN033 genome identified two putative vgrGs genes (vgrG1 and 0248) located inside T6SS cluster and another two (vgrG2 and 1588) outside it. This study determined the function of the four putative VgrG proteins by constructing a series of mutants and complemented strains. In vitro, the VgrG1 protein was observed to be involved in the antibacterial ability and the interactions with cells. The animal model experiment showed that the deletion of vgrG1 significantly led to the decrease in the multiplication capacity of PCN033. However, the deletion of 0248 and/or the deletion of vgrG2 and 1588 had no effect on the pathogenicity of PCN033. The study of four putative VgrGs in PCN033 indicated that only VgrG1 plays an important role in the interaction between PCN033 and other bacteria or host cells. This study can provide a novel perspective to the pathogenesis of PCN033 and lay the foundation for discovering potential T6SS effectors.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：湖北省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台